Relationship of neuronal nitric oxide synthase immunoreactivity to GnRH neurons in the ovariectomized and intact female rat
Relationship of neuronal nitric oxide synthase immunoreactivity to GnRH neurons in the ovariectomized and intact female rat
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DOI：
10.1111/j.1365-2826.1996.tb00688.x
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发表时间：
1996-01-01
影响因子：
3.2
通讯作者：
Emson, PC
中科院分区：
文献类型：
--
作者：
Herbison, AE;Simonian, SX;Emson, PC
本研究使用大鼠神经元型一氧化氮合酶（nNOS）抗体，检测去卵巢以及处于间情期和动情前期的完整大鼠中nNOS免疫反应性与促性腺激素释放激素（GnRH）神经元的关系。在视前区前部发现了一条显著的nNOS免疫反应细胞带，它始于视前正中核和终板血管器，并在室周前腹核水平的第三脑室前部上方形成倒Y型分布。还发现另一条nNOS免疫反应带穿过正中隆起的内侧区延伸至弓状核。尽管在任何实验组的GnRH神经元胞体中均未检测到nNOS免疫反应性，但发现位于视前区前部（而非其他部位）的GnRH神经元胞体被含nNOS的细胞所包围。在正中隆起中，nNOS和GnRH免疫反应性分别分布在其内侧区和外侧区。这些结果证明，无论其活动模式如何，雌性大鼠的GnRH神经元均不表达nNOS。相反，在视前区前部的特定亚区以及正中隆起中，已确定nNOS免疫反应细胞与GnRH神经元胞体和纤维之间存在密切的解剖学关系。这些发现与一氧化氮在这两个部位在整个发情周期中调节GnRH释放的作用相符。
The present study has used a rat neuronal nitric oxide synthase (nNOS) antibody to examine the relationship of nNOS immunoreactivity to GnRH neurons in the ovariectomized and intact diestrous and proestrous rat. A striking band of nNOS-immunoreactive cells was identified in the rostral preoptic area which began in the median preoptic nucleus and organum vasculosum of the lamina terminalis and formed an inverted Y-type distribution above the rostral third ventricle at the level of the anteroventral periventricular nucleus. Another band of nNOS-immunoreactivity was found extending through the internal zone of the median eminence into the arcuate nucleus. Although nNOS immunoreactivity was not detected within GnRH neuronal cell bodies in any of the experimental groups, GnRH perikarya located in the rostral preoptic area, but not elsewhere, were found to be surrounded by nNOS-containing cells, In the median eminence, nNOS and GnRH immunoreactivities were distributed separately in the internal and external zones, respectively.These results provide evidence that, regardless of their pattern of activity, GnRH neurons in the female rat do not express nNOS. Instead, a close anatomical relationship between nNOS-immunoreactive cells and GnRH perikarya and fibers has been identified within specific sub-regions of the rostral preoptic area and in the median eminence. Such findings are compatible with a role for NO at both sites in regulating the release of GnRH throughout the estrous cycle.