Conserved regulatory elements in the promoter region of the N-CAM gene.
Conserved regulatory elements in the promoter region of the N-CAM gene.
复制标题
N-CAM 基因启动子区的保守调控元件。
DOI：
10.1016/s0888-7543(05)80108-9
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发表时间：
1992
期刊：
影响因子：
4.4
通讯作者：
Brackenbury,R
中科院分区：
文献类型：
--
作者：
Colwell,G;Li,B;Forrest,D;Brackenbury,R
通过限制性图谱和 DNA 测序对含有鸡 N-CAM 基因 5' 侧翼序列、第一个外显子和整个第一个内含子的基因组克隆进行了表征。包含第一个外显子的 > 600 bp 片段非常富含 G + C，并且包含大量 CpG 二核苷酸，表明它代表 CpG 岛。存在 SP-1 和 AP-1 共有元件，但在第一个外显子上游 300 bp 内未发现 TATA 或 CCAAT 样元件。将鸡启动子区域序列与人类、大鼠和小鼠 N-CAM 基因的相似区域进行比较，结果表明，一些潜在的调控元件，包括小鼠和大鼠 N-CAM 基因中的“嘌呤盒”、哺乳动物 N-CAM 基因中的两个同源域结合区之一以及几个潜在的 SP-1 位点，在该区域内并不保守。相反，高 CpG 含量、同源域结合序列、SP-1 元件、八聚体元件和 AP-1 元件在所有四个基因中都是保守的。鸡基因的第一个内含子为 38 kb，远小于哺乳动物 N-CAM 基因的相应内含子。结合前期研究，本工作完成了鸡N-CAM基因的克隆，该基因包含至少26个外显子，分布在85 kb以上。
Genomic clones containing 5′-flanking sequences, the first exon, and the entire first intron from the chicken N-CAM gene were characterized by restriction mapping and DNA sequencing. A > 600-bp segment that includes the first exon is very G + C-rich and contains a large proportion of CpG dinucleotides, suggesting that it represents a CpG island. SP-1 and AP-1 consensus elements are present, but no TATA- or CCAAT-like elements were found within 300 bp upstream of the first exon. Comparison of the chicken promoter region sequence with similar regions of the human, rat, and mouse N-CAM genes revealed that some potential regulatory elements including a “purine box” seen in mouse and rat N-CAM genes, one of two homeodomain binding regions seen in mammalian N-CAM genes, and several potential SP-1 sites are not conserved within this region. In contrast, high CpG content, a homeodomain binding sequence, an SP-1 element, an octomer element, and an AP-1 element are conserved in all four genes. The first intron of the chicken gene is 38 kb, substantially smaller than the corresponding intron from mammalian N-CAM genes. Together with previous studies, this work completes the cloning of the chicken N-CAM gene, which contains at least 26 exons distributed over 85 kb.