A BODIPY-Based Donor/Donor-Acceptor System: Towards Highly Efficient Long-Wavelength-Excitable Near-IR Polymer Dots with Narrow and Strong Absorption Features
A BODIPY-Based Donor/Donor-Acceptor System: Towards Highly Efficient Long-Wavelength-Excitable Near-IR Polymer Dots with Narrow and Strong Absorption Features
复制标题
基于 BODIPY 的供体/供体-受体系统：实现具有窄而强吸收特性的高效长波长可激发近红外聚合物点
DOI：
10.1002/anie.201902077
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发表时间：
2019-05-20
影响因子：
16.6
通讯作者：
Chiu, Daniel T.
中科院分区：
文献类型：
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作者：
Chen, Lei;Chen, Dandan;Chiu, Daniel T.
明亮的长波长可激发半导体聚合物点（LWE-Pdot）是体内成像和多路复用体外生物测定非常需要的。通过将近红外（NIR）发射体并入聚合物主体的主链中以开发二元供体-受体（D-A）系统来获得LWE-Pdot。然而，它们通常遭受严重的浓度猝灭和荧光量子产率（Phi（f））和吸收截面（sigma）之间的权衡。本文中，我们描述了一种三元组分（D-1/D-2-A）策略，通过引入基于BODIPY的辅助聚合物供体作为D-1来实现具有窄带NIR发射的超亮、绿色激光可激发的Pdot。与相应的二进制D-2-A Pdot相比，D-1/D-2-A Pdot具有改进的Phi（f）和sigma。它们的Phi（f）高达40.2%，是报道的最有效的近红外Pdot之一。D-1/D-2-A Pdot在532 nm激光激发下显示出比Qdot 705亮83倍的单粒子亮度。当注射到小鼠中时，使用三元Pdot相对于二元D-A Pdot实现了更高对比度的体内肿瘤成像。
Bright long-wavelength-excitable semiconducting polymer dots (LWE-Pdots) are highly desirable for invivo imaging and multiplexed invitro bioassays. LWE-Pdots have been obtained by incorporating a near-infrared (NIR) emitter into the backbone of a polymer host to develop a binary donor-acceptor (D-A) system. However, they usually suffer from severe concentration quenching and a trade-off between fluorescence quantum yield (Phi(f)) and absorption cross-section (sigma). Herein, we describe a ternary component (D-1/D-2-A) strategy to achieve ultrabright, green laser-excitable Pdots with narrow-band NIR emission by introducing a BODIPY-based assistant polymer donor as D-1. The D-1/D-2-A Pdots possess improved Phi(f) and sigma compared to corresponding binary D-2-A Pdots. Their Phi(f) is as high as 40.2%, one of the most efficient NIR Pdots reported. The D-1/D-2-A Pdots show ultrahigh single-particle brightness, 83-fold brighter than Qdot 705 when excited by a 532nm laser. When injected into mice, higher contrast invivo tumor imaging was achieved using the ternary Pdots versus the binary D-A Pdots.