Peritoneal mesothelium promotes the progression of ovarian cancer cells in vitro and in a mice xenograft model in vivo
Peritoneal mesothelium promotes the progression of ovarian cancer cells in vitro and in a mice xenograft model in vivo
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DOI：
10.1016/j.canlet.2014.09.041
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发表时间：
2014-12-28
期刊：
影响因子：
9.7
通讯作者：
Ksiazek, Krzysztof
中科院分区：
文献类型：
--
作者：
Mikula-Pietrasik, Justyna;Sosinska, Patrycja;Ksiazek, Krzysztof
间皮细胞在卵巢癌腹膜内扩散中的作用仍不清楚。特别是，目前还不清楚这些细胞是否构成了阻止癌细胞发展的被动屏障，或者可能是这一过程的积极推动者。在本报告中，我们发现人腹膜间皮细胞(HPMCs)能刺激卵巢癌细胞(A2780、OVCAR-3、SKOV-3)的黏附和增殖。后者与HPMCs向环境释放的Gro-1、IL-6和IL-8的旁分泌活性有关。此外，对卵巢癌异种移植瘤的生长动力学进行了研究。卵巢癌细胞与HPMCs联合注射明显多于单独移植癌细胞。在每一种异种移植模型中，腹膜间皮细胞始终存在于肿瘤块附近。综上所述，我们的结果表明，HPMCs在卵巢恶性肿瘤的腹膜内侵袭中起支持作用，其作用可能与其刺激癌细胞黏附和增殖的能力有关。(C)2014爱思唯尔爱尔兰有限公司。保留所有权利。
The role of mesothelial cells in the intraperitoneal spread of ovarian cancer is still elusive. In particular, it is unclear whether these cells constitute a passive barrier preventing cancer cell progression or perhaps act as an active promoter of this process. In this report we show that omental human peritoneal mesothelial cells (HPMCs) stimulate adhesion and proliferation of ovarian cancer cells (A2780, OVCAR-3, SKOV-3). The latter was associated with the paracrine activity of GRO-1, IL-6, and IL-8 released to the environment by HPMCs. Furthermore, the growth dynamics of ovarian cancer xenografts produced in response to i.p. injection of ovarian cancer cells together with HPMCs was remarkably greater than for implantation of cancer cells alone. A layer of peritoneal mesothelium was consistently present in close proximity to the tumor mass in every xenograft model. In conclusion, our results indicate that HPMCs play a supporting role in the intraperitoneal invasiveness of ovarian malignancy, whose effect may be attributed to their ability to stimulate adhesion and proliferation of cancer cells. (C) 2014 Elsevier Ireland Ltd. All rights reserved.