Three-dimensional imaging of microvasculature in the rat spinal cord following injury.
Three-dimensional imaging of microvasculature in the rat spinal cord following injury.
复制标题
大鼠脊髓损伤后微血管的三维成像
DOI：
10.1038/srep12643
复制
发表时间：
2015-07-29
影响因子：
4.6
通讯作者：
Lu H
中科院分区：
文献类型：
--
作者：
Cao Y;Wu T;Yuan Z;Li D;Ni S;Hu J;Lu H
研究损伤后脊髓微血管的三维（3D）形态学改变提供了对脊髓损伤（SCI）病理学的深入了解。过去，该领域的知识受到成像技术的阻碍，该技术仅提供关于血管对创伤的反应的二维（2D）信息。本研究的目的是利用同步辐射显微断层扫描（SRμCT）研究大鼠脊髓损伤后第1天微血管的三维显微结构变化。该技术提供了正常和受损脊髓中微血管的高分辨率3D图像，检测到的最小血管约为7.4 μm。此外，我们优化了三维血管可视化与颜色编码，并准确地计算定量变化的血管结构后SCI。与对照脊髓相比，损伤后脊髓血管数目明显减少。此外，损伤区域并不集中在震中;相反，损伤的迹象在3D中沿着脊髓向头侧和尾侧沿着。组织学检查也证实了观察到的病理变化。这些结果表明，SRμCT是一种有效的技术平台，可用于神经血管疾病小动脉病变的成像和治疗干预措施的评价。
Research studies on the three-dimensional (3D) morphological alterations of the spinal cord microvasculature after injury provide insight into the pathology of spinal cord injury (SCI). Knowledge in this field has been hampered in the past by imaging technologies that provided only two-dimensional (2D) information on the vascular reactions to trauma. The aim of our study is to investigate the 3D microstructural changes of the rat spinal cord microvasculature on day 1 post-injury using synchrotron radiation micro-tomography (SRμCT). This technology provides high-resolution 3D images of microvasculature in both normal and injured spinal cords and the smallest vessel detected is approximately 7.4 μm. Moreover, we optimized the 3D vascular visualization with color coding and accurately calculated quantitative changes in vascular architecture after SCI. Compared to the control spinal cord, the damaged spinal cord vessel numbers decreased significantly following injury. Furthermore, the area of injury did not remain concentrated at the epicenter; rather, the signs of damage expanded rostrally and caudally along the spinal cord in 3D. The observed pathological changes were also confirmed by histological tests. These results demonstrate that SRμCT is an effective technology platform for imaging pathological changes in small arteries in neurovascular disease and for evaluating therapeutic interventions.