Heme Signaling Impacts Global Gene Expression, Immunity and Dengue Virus Infectivity in Aedes aegypti.
Heme Signaling Impacts Global Gene Expression, Immunity and Dengue Virus Infectivity in Aedes aegypti.
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DOI：
10.1371/journal.pone.0135985
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Paiva-Silva GO
中科院分区：
文献类型：
--
作者：
Bottino-Rojas V;Talyuli OA;Jupatanakul N;Sim S;Dimopoulos G;Venancio TM;Bahia AC;Sorgine MH;Oliveira PL;Paiva-Silva GO
吸血的蚊子暴露在高水平的血红素中，血红素是血红蛋白降解的产物。血红素是一种促氧化剂，影响多种细胞过程。我们对血红素调控的埃及伊蚊（黄热病蚊子）转录变化进行了全球分析，以便在分子水平上更好地了解对蚊子生理的影响。我们观察到血红素诱导的不依赖铁和活性氧（ROS）的信号，其中包括与氧化还原代谢相关的基因。通过调节这些转录本的丰度，血红素可能作为一种危险的信号分子。此外，血红素引发了能量代谢和免疫应答基因表达的关键变化，改变了对细菌和登革热病毒的易感性。这些发现似乎暗示了蚊子对吸血的适应，从而影响了它们传播疾病的能力。总之，这些结果也可能有助于了解真核细胞中的血红素细胞生物学。
Blood-feeding mosquitoes are exposed to high levels of heme, the product of hemoglobin degradation. Heme is a pro-oxidant that influences a variety of cellular processes. We performed a global analysis of heme-regulated Aedes aegypti (yellow fever mosquito) transcriptional changes to better understand influence on mosquito physiology at the molecular level. We observed an iron- and reactive oxygen species (ROS)-independent signaling induced by heme that comprised genes related to redox metabolism. By modulating the abundance of these transcripts, heme possibly acts as a danger signaling molecule. Furthermore, heme triggered critical changes in the expression of energy metabolism and immune response genes, altering the susceptibility towards bacteria and dengue virus. These findings seem to have implications on the adaptation of mosquitoes to hematophagy and consequently on their ability to transmit diseases. Altogether, these results may also contribute to the understanding of heme cell biology in eukaryotic cells.