Mapping of the RNA promoter of Newcastle disease virus
Mapping of the RNA promoter of Newcastle disease virus
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DOI：
10.1016/j.virol.2004.10.040
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发表时间：
2005-01-20
期刊：
影响因子：
3.7
通讯作者：
Villar, E
中科院分区：
文献类型：
--
作者：
Marcos, F;Ferreira, L;Villar, E
通过对纽卡斯尔病毒（NDV）基因组和反基因组RNA启动子3'末端的突变分析，对NDV基因组和反基因组RNA启动子进行了研究。与其他副粘病毒相似，NDV RNA复制遵循六规则，基因组和反基因组启动子需要两个不连续的区域：保守区1（前18个核苷酸）和保守区II（核苷酸73-90）。这些区域之间的适当间隔和区域11中的六个相位对于有效的RNA启动子活性至关重要。正如预期的，NDV基因组3'末端的基因起始信号是mRNA转录所需的，但不是RNA复制所需的。令人惊讶的是，5'端多聚腺苷酸化信号的突变不影响基因表达转录。虽然NDV（avulavirus）启动子的保守区1看起来更类似于仙台病毒（SeV）（呼吸道病毒），但保守区II类似于风疹病毒。(C)2004年爱思唯尔公司All rights reserved.
The RNA promoters of the genome and antigenome of Newcastle disease virus (NDV) were studied by mutational analysis of their 3' terminal ends. Similarly to other paramyxoviruses, NDV RNA replication follows the rule of six, and the genomic and antigenomic promoters require two discontinuous regions: conserved region 1 (first 18 nucleotides) and conserved region II (nucleotides 73-90). Proper spacing between those regions and the phase of six in region 11 is critical for efficient RNA promoter activity. As expected, the gene start signal at the 3' end of the NDV genome was required for mRNA transcription, but not for RNA replication. Surprisingly, mutation of the polyadenylation signal in the 5' end did not affect gene expression transcription. Although the conserved region 1 of NDV (avulavirus) promoter appears to be more similar to that of Sendai virus (SeV) (respirovirus), conserved region II is analogous to that of rubulaviruses. (C) 2004 Elsevier Inc. All rights reserved.