Effects of Magnoline on P-Selectin's Expression in Diabetic Rats and its Reno-Protection
Effects of Magnoline on P-Selectin's Expression in Diabetic Rats and its Reno-Protection
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DOI：
10.1159/000350146
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发表时间：
2013-01-01
影响因子：
2.8
通讯作者：
Wang, Niansong
中科院分区：
文献类型：
--
作者：
Zhou, Yang;Wang, Feng;Wang, Niansong
背景与目的：木兰碱是中药巴山木垒的有效成分，具有抗炎和抗血小板作用。目的探讨木兰碱对糖尿病大鼠肾脏的保护作用及其对P-选择素的影响。研究方法：将36只大鼠随机分为4组，即正常对照组（C）、糖尿病组（D）、木兰脂素小剂量治疗组（M1）和木兰脂素大剂量治疗组（M2），每组n=9）。选用链脲佐菌素建立糖尿病大鼠模型，M1组和M2组分别给予木兰碱0.5mg/Kg·d和2 mg/Kg·d。治疗16周后观察尿白蛋白排泄率、肾功能、P-选择素和TGF-β 1水平。结果如下：M1组尿蛋白和血清肌酐水平（1078.9 +/- 77.3 μ g/24 h，29.7 +/- 3.9 μ mol/L）和M2（852.9 ± 80.1 μ g/24h，30.9 ± 2.9 μ mol/L）低于D组（1572.8 ± 176.2 μ g/24h，39.4 ± 4.1 μ mol/L）（P < 0.05）。M1、M2组血清P-选择素水平低于D组（P < 0.05）。M1、M2组肾组织P-选择素和TGF-β 1表达均明显减弱。结论：木兰碱对糖尿病大鼠肾脏有保护作用，其机制可能与抑制P-选择素有关。版权所有（C）2013 S. Karger AG，巴塞尔
Background and Objective: Magnoline is an active ingredient of magnolia fargesii with anti-inflammatory and anti-platelet effects. The objective is to explore the renoprotection of magnoline in diabetic rats and its effects on P-selectin. Methods: Thirty-six rats were randomized into 4 groups-normal control group (C), diabetic group (D), small-dose magnoline treatment group (M1) and large-dose magnoline treatment group (M2) (n=9 in each group). Streptozotocin was selected to construct diabetic rat model, and group M1 and group M2 were treated with magnoline 0.5mg/Kg.d and 2mg/Kg.d respectively. Urinary albumin excretion rate, renal function, levels of P-selectin and TGF-beta 1 were observed after 16 weeks. Results: Levels of albuminuria and serum creatinine of group M1 (1078.9 +/- 77.3 mu g/24h, 29.7 +/- 3.9 mu mol/L) and M2 (852.9 +/- 80.1 mu g/24h, 30.9 +/- 2.9 mu mol/L) were lower than group D (1572.8 +/- 176.2 mu g/24h, 39.4 +/- 4.1 mu mol/L) (P < 0.05). Serum levels of P-selectin in group M1 and M2 were lower than group D (P < 0.05). The renal expression of P-selectin and TGF-beta 1 in group M1 and M2 were significantly attenuated respectively. Conclusions: Magnoline has reno-protective effects on diabetic rats which may be related to the inhibition of P-selectin. Copyright (C) 2013 S. Karger AG, Basel