Mitochondrial oxygen consumption rate of human embryos declines with maternal age
Mitochondrial oxygen consumption rate of human embryos declines with maternal age
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DOI：
10.1007/s10815-020-01869-5
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发表时间：
2020-08-01
影响因子：
3.1
通讯作者：
Shibahara, Hiroaki
中科院分区：
文献类型：
--
作者：
Morimoto, Naoharu;Hashimoto, Shu;Shibahara, Hiroaki
研究目的女性的生育能力随着年龄的增长而下降，这是由于非整倍体发生率的增加和卵母细胞中线粒体活性的降低等因素造成的。然而，母体衰老与其胚胎线粒体功能之间的关系仍然未知。在这里，我们评估了母亲的年龄和线粒体功能之间的关系，在他们的卵母细胞和胚胎方法之间的关系，母亲的年龄和耗氧率（OCRs），线粒体DNA（mtDNA）拷贝数，或囊胚发育进行了调查，使用81个胚胎从63对不孕夫妇。回顾性分析105例患者的桑椹胚至囊胚发育率。结果桑椹胚各期mtDNA拷贝数与母亲年龄无关，但随着母亲年龄的增加，桑椹胚的OCRs逐渐降低（r（2）= 0.48，P < 0.05）。桑椹胚期耗氧量越高，胚胎发育至中期囊胚所需时间越短（r（2）= 0.236，P < 0.05）。根据临床数据分析，从桑椹胚到囊胚的发育率随着母亲年龄的增加而降低（P< 0.05，< 37岁，81.1%;>= 37岁，64.1%）。结论人胚胎桑椹胚期线粒体功能随母体年龄增长而下降，线粒体功能下降导致桑椹胚发育缓慢，发育率降低。
Purpose The fertility of women decreases with age because of factors such as an increased incidence of aneuploidies and-possibly-decreased mitochondrial activity in oocytes. However, the relationship between maternal aging and mitochondrial function of their embryos remains unknown. Here, we assessed the relationship between maternal age and mitochondrial functions in their oocytes and embryos Methods The relationships between maternal age and oxygen consumption rates (OCRs), mitochondrial DNA (mtDNA) copy numbers, or blastocyst development was investigated using 81 embryos donated from 63 infertility couples. The developmental rates from morulae to blastocysts were retrospectively analyzed using data of 105 patients. Results The OCRs of morulae decreased with maternal age (r(2)= 0.48, P < 0.05) although there were no relationships between maternal age and mtDNA copy number in any stages. The more oxygen consumed at the morula stage, the shorter time was required for embryo development to the mid-stage blastocyst (r(2)= 0.236,P< 0.05). According to the clinical data analysis, the developmental rate from morulae to blastocysts decreased with maternal age (P< 0.05, < 37 years, 81.1%, vs. >= 37 years, 64.1%). Conclusions The data of the present study revealed that mitochondrial function at the morula stage of human embryos decreased with their maternal age and a decrease of mitochondrial function led to slow-paced development and impaired developmental rate from morulae to blastocysts.