Microbe-focused glycan array screening platform
Microbe-focused glycan array screening platform
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DOI：
10.1073/pnas.1800853116
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发表时间：
2019-02-05
影响因子：
11.1
通讯作者：
Seeberger, Peter H.
中科院分区：
文献类型：
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作者：
Geissner, Andreas;Reinhardt, Anika;Seeberger, Peter H.
聚糖和聚糖结合蛋白之间的相互作用在所有生命领域的许多过程中都是必不可少的。聚糖微阵列是检测蛋白质-聚糖相互作用的极好工具。在这里，我们提出了一个基于化学合成获得的低聚糖的微生物聚糖微阵列平台。多糖是通过结合不同的碳水化合物合成方法生成的，包括自动聚糖组装、液相合成和化学酶法。目前超过300个聚糖库与来自功能糖组学协会的哺乳动物聚糖阵列一样多样化，并且由于其微生物重点，高度互补。这种聚糖平台对于表征各种类型的聚糖结合蛋白是必不可少的。该聚糖阵列平台的应用重点是先天免疫受体和细菌毒力因子的表征以及人类对致病性聚糖的体液免疫分析。
Interactions between glycans and glycan binding proteins are essential for numerous processes in all kingdoms of life. Glycan microarrays are an excellent tool to examine protein-glycan interactions. Here, we present a microbe-focused glycan microarray platform based on oligosaccharides obtained by chemical synthesis. Glycans were generated by combining different carbohydrate synthesis approaches including automated glycan assembly, solution-phase synthesis, and chemoenzymatic methods. The current library of more than 300 glycans is as diverse as the mammalian glycan array from the Consortium for Functional Glycomics and, due to its microbial focus, highly complementary. This glycan platform is essential for the characterization of various classes of glycan binding proteins. Applications of this glycan array platform are highlighted by the characterization of innate immune receptors and bacterial virulence factors as well as the analysis of human humoral immunity to pathogenic glycans.