THIN-LAYER CHROMATOGRAPHY OF CARBOHYDRATES IN PRESENCE OF BISULFITE
THIN-LAYER CHROMATOGRAPHY OF CARBOHYDRATES IN PRESENCE OF BISULFITE
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DOI：
10.1016/s0021-9673(00)99871-6
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发表时间：
1965-01-01
期刊：
影响因子：
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通讯作者：
ADACHI, S
中科院分区：
文献类型：
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作者：
ADACHI, S
本文提出了一种用硅胶G-少量亚硫酸氢钠薄层色谱快速分离糖类的方法。溶剂系统丙醇-水（85：15）给出了最好的分离的碳水化合物，但没有分离一些戊糖。采用邻氨基二苯基磷酸、咔唑-硫酸、双甲酮-磷酸、苯酚-硫酸和百里酚-硫酸五种喷雾试剂测定糖类化合物。除咔唑-硫酸试剂外，小至0.1 μg的痕量镉都能用这些试剂轻易地检测出来。碳水化合物与试剂一起呈现特征颜色，从而使识别更容易。
A rapid method for separating carbohydrates by means of thin-layer chromatography on silica gel G mixed with a small amount of sodium bisulfite has been developed. The solvent system propanol—water (85:15) gave the best resolution of the carbohydrates but did not separate some pentoses. Five spray reagents,viz.o-amino-diphenyl—orthophosphoric acid, carbazole—sulfuric acid, dimedone—orthophosphoric acid, phenol—sulfuric acid and thymol—sulfuric acid, were used for the detection of carbohydrates. Quantities as small as 0.1 μg could be easily detected with these reagents, with one exception,viz.carbazole—sulfuric acid reagent. The carbohydrates gave characteristic colors with the reagents, thus making identification easier.