The advancing identification and classification of Rhizoctonia spp. using molecular and biotechnological methods compared with the classical anastomosis grouping
The advancing identification and classification of Rhizoctonia spp. using molecular and biotechnological methods compared with the classical anastomosis grouping
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DOI：
10.1007/s10267-006-0320-x
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发表时间：
2006-12-01
期刊：
影响因子：
1.4
通讯作者：
Sneh, Baruch
中科院分区：
文献类型：
--
作者：
Sharon, Michal;Kuninaga, Shiro;Sneh, Baruch
丝核菌的无性型分类。除了有性型分类外，还基于年轻细胞核数和菌丝融合群（AG）。分子生物学技术的广泛发展增加了现代工具，以支持根据其遗传学和进化过程对生物体进行分类。近年来，这些不同的方法也被用于丝核菌的分类。数据在文献和数据库中不断积累，这些数据在网络系统中随时可供研究人员使用。本文综述了丝核菌分类的各种方法，并对各种方法的优缺点进行了比较。目前，rDNA-内转录间隔区（ITS）序列分析似乎是丝核菌分类的最合适的方法。利用邻接树（NJ）和最大简约树（NIP）分析了GenBank中积累的所有多核丝核菌（MNR）的数据，并补充了AGs和亚群内部和之间的序列相似性。一般来说，分离物序列的聚类支持基于菌丝融合吻合的AG和亚组。本文还指出了GenBank中某些分离株序列命名的不准确性。该综述包括MNR组和亚组的详细分析，而双核丝核菌（BNR），单核丝核菌（UNR）的补充说明，以及所有目前可用的MNR，BNR和UNR组和亚组之间的全面的相互关系，在随后的评论文章中进行了讨论。
Anamorphic classification of Rhizoctonia spp. has been based on young cell nuclear numbers and hyphal fusion to anastomosis groups (AGs), in addition to the teleomorph classification. The widespread development of molecular biology techniques has added modern tools to support classification of organisms according to their genetics and evolutionary processes. These various methods have also been used in recent years for classification of Rhizoctonia. Data are continuously accumulating in the literature and the sequences in databases, which are readily available for researchers in the network systems. In the present review, attempts were made to describe and compare the advantages and disadvantages of the various methods for the classification of Rhizoctonia spp. Currently, the rDNA-internal transcribed spacer (ITS) sequence analysis seems to be the most appropriate method for classification of Rhizoctonia spp. Data of all the appropriate multinucleate Rhizoctonia (MNR) accumulated in GenBank were analyzed together in neighbor-joining (NJ) and maximum-parsimony (NIP) trees supplemented with percent sequence similarity within and among AGs and subgroups. Generally, the clusters of the isolate sequences were supportive of the AGs and subgroups based on hyphal fusion anastomosis. The review also indicates inaccuracies in designation of sequences of some isolates deposited in GenBank. The review includes detailed analyses of the MNR groups and subgroups, whereas complementary descriptions of the binucleate Rhizoctonia (BNR), uninucleate Rhizoctonia (UNR), and comprehensive interrelationships among all the currently available MNR, BNR, and UNR groups and subgroups in GenBank are to be discussed in a subsequent review article.