Effect of Codon Message on Xylanase Thermal Activity
Effect of Codon Message on Xylanase Thermal Activity
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密码子信息对木聚糖酶热活性的影响
DOI：
10.1074/jbc.m111.327577
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发表时间：
2012-08-03
影响因子：
4.8
通讯作者：
Chen, Hongge
中科院分区：
文献类型：
--
作者：
Liu, Liangwei;Wang, Linmin;Chen, Hongge
由于遗传密码子是众所周知的简并性，它对酶热性质的影响很少被研究。构建了GH10木聚糖酶编码序列和最适酶活温度(T-opt)的数据集。计算了木聚糖酶的密码子含量和相对同义密码子用法，并分别与描述木聚糖酶嗜热倾向的酶T-opt值进行了相关，而不将其分为嗜热和中温两类。对密码子含量和相对同义密码子用法进行Bonferroni校正法检验后，我们发现了5个密码子，其中3个密码子(AUA、AGA和AGG)与T-opt值呈正相关，2个(CGU和AGC)与T-opt值呈负相关。3个正密码子为富嘌呤密码子，2个负密码子为A-端。两个负密码子是富含吡啶的密码子，其中一个有C-端。与密码子C-和A-末端特征类似，基因中C-和A-含量分别与T-opt值呈负相关和正相关。因此，密码子对酶的热性质有影响。当在残差水平上分析问题时，密码子消息的效果就会丢失。与酶热性质有关的密码子是通过核苷酸水平上的嗜热力来选择的。
Because the genetic codon is known for degeneracy, its effect on enzyme thermal property is seldom investigated. A dataset was constructed for GH10 xylanase coding sequences and optimal temperatures for activity (T-opt). Codon contents and relative synonymous codon usages were calculated and respectively correlated with the enzyme T-opt values, which were used to describe the xylanase thermophilic tendencies without dividing them into two thermophilic and mesophilic groups. After analyses of codon content and relative synonymous codon usages were checked by the Bonferroni correction, we found five codons, with three (AUA, AGA, and AGG) correlating positively and two (CGU and AGC) correlating negatively with the T-opt value. The three positive codons are purine-rich codons, and the two negative codons have A-ends. The two negative codons are pyridine-rich codons, and one has a C-end. Comparable with the codon C- and A-ending features, C- and A-content within mRNA correlated negatively and positively with the T-opt value, respectively. Thereby, codons have effects on enzyme thermal property. When the issue is analyzed at the residual level, the effect of codon message is lost. The codons relating to enzyme thermal property are selected by thermophilic force at nucleotide level.