SEQUENCE AND DOMAIN-STRUCTURE OF TALIN
SEQUENCE AND DOMAIN-STRUCTURE OF TALIN
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DOI：
10.1038/347685a0
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发表时间：
1990-10-18
期刊：
影响因子：
64.8
通讯作者：
HYNES, RO
中科院分区：
文献类型：
--
作者：
REES, DJG;ADES, SE;HYNES, RO
Talin是一种高相对分子质量的细胞骨架蛋白，主要集中在细胞-基质接触区1和淋巴细胞中的细胞-细胞接触区2，3。整合素受体参与贴壁细胞与细胞外基质4，5和淋巴细胞与其他细胞6的附着。在这些情况下，Talin与细胞表面膜上的整合素浓度共分布3，7-9。此外，在体外结合研究中表明，整合素与talin结合，尽管亲和力较低10。Talin还与vinculin11高亲和力结合，vinculin11是另一种细胞骨架蛋白，集中在细胞粘附点。最后，Talin是钙激活的蛋白水解酶CalPain II13，14的底物，它也集中在细胞-底物接触点14。为了更多地了解talin的结构及其参与细胞外粘连和细胞骨架之间的跨膜连接，我们克隆了小鼠talin并对其进行了测序。我们描述了一个基于该序列和其他数据的Talin结构模型。Talin和其他蛋白之间的同源性定义了一个新的膜下细胞骨架相关蛋白家族，所有这些蛋白显然都参与了与质膜的连接。
TALIN is a high-molecular-weight cytoskeletal protein concentrated at regions of cell–substratum contact1and, in lymphocytes, at cell–cell contacts2,3. Integrin receptors are involved in the attachment of adherent cells to extracellular matrices4,5and of lymphocytes to other cells6. In these situations, talin codistributes with concentrations of integrins in the cell surface membrane3,7–9. Furthermore,in vitrobinding studies suggest that integrins bind to talin, although with low affinity10. Talin also binds with high affinity to vinculin11, another cytoskeletal protein concentrated at points of cell adhesion12. Finally, talin is a substrate for the Ca2+-activated protease, calpain II13,14, which is also concentrated at points of cell-substratum contact14. To learn more about the structure of talin and its involvement in transmembrane connections between extracellular adhesions and the cytoskeleton, we have cloned and sequenced murine talin. We describe a model for the structure of talin based on this sequence and other data. Homologies between talin and other proteins define a novel family of submembranous cytoskeleton-associated proteins all apparently involved in connections to the plasma membrane.