A review of non-invasive optical-based image analysis systems for continuous bioprocess monitoring
A review of non-invasive optical-based image analysis systems for continuous bioprocess monitoring
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DOI：
10.1007/s00449-009-0319-8
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发表时间：
2010-02-01
影响因子：
3.8
通讯作者：
Scheper, Thomas
中科院分区：
文献类型：
--
作者：
Hoepfner, Tim;Bluma, Arne;Scheper, Thomas
为了观察和控制栽培过程，光学传感器被越来越多地使用。控制这一过程的重要变量是细胞计数、细胞大小分布和细胞形态。在浊度测量方法中，成像程序用于确定这些过程值。以前开发的大多数成像程序的一个缺点是，它们只能离线使用，这需要采样。另一方面，到目前为止，可用的成像在线探头只能提供有限数量的过程价值。这篇文章概述了在线确定细胞计数、细胞大小分布和其他变量的光学程序。具体地说，通过原位显微镜，将描述一种成像程序，其允许在不采样的情况下实时确定直接和非直接细胞变量。
To observe and control cultivation processes, optical sensors are used increasingly. Important variables for controlling such processes are cell count, cell size distribution and the morphology of cells. Among turbidity measurement methods, imaging procedures are applied for determining these process values. A disadvantage of most previously developed imaging procedures is that they are only available offline, which requires sampling. On the other hand, available imaging inline probes can only deliver a limited number of process values so far. This contribution gives an overview of optical procedures for the inline determination of cell count, cell size distribution and other variables. In particular, by in situ microscopy, an imaging procedure will be described, which allows the determination of direct and non-direct cell variables in real time without sampling.