Organisation of trypsin genes in the salmon louse (Lepeophtheirus salmonis, Crustacea, copepoda) genome
Organisation of trypsin genes in the salmon louse (Lepeophtheirus salmonis, Crustacea, copepoda) genome
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DOI：
10.1016/j.gene.2005.03.011
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发表时间：
2005-06-06
期刊：
影响因子：
3.5
通讯作者：
Nilsen, F
中科院分区：
文献类型：
--
作者：
Kvamme, BO;Kongshaug, H;Nilsen, F
胰蛋白酶构成丝氨酸肽酶S1 A家族的一个亚类，存在于所有动物和一些细菌中。目前，没有关于桡足类胰蛋白酶基因组结构的信息。桡足类胰蛋白酶的唯一数据表明，海洋寄生桡足类Lepeophtheirus salmonis中存在几种不同的胰蛋白酶。在本研究中，31.7 kbp的基因组DNA周围的先前描述的LsTryp 1 -5序列进行测序。测序区域包含9个全长和3个部分胰蛋白酶基因。根据PCR分析和基因组序列保守估计，L.鲑鱼，其中18个与先前描述的LsTyp 1和LsTyp 2 cDNA序列最相似。这些基因中有四个是假定的假基因。此外，还鉴定了一个推测的水手样转座酶基因。基因组序列表明L.鲑胰蛋白酶基因存在于一个或多个基因簇内。三种不同的LsTryp内含子外显子结构被确定，所有这三个是不同的内含子外显子组织先前报道的其他S1 A肽酶。这意味着在L.鲑鱼胰蛋白酶基因。(c)2005 Elsevier B. V.保留所有权利。
Trypsins constitute a subclass of the S1A family of serine peptidases found in all groups of animal and some bacteria. At present, no information about the genomic organisation of trypsins is available for copepods. The only data of copepod trypsins indicate several different trypsins in the marine parasitic copepod Lepeophtheirus salmonis. In the present study, 31.7 kbp of genomic DNA surrounding the previously described LsTryp1-5 sequences was sequenced. The sequenced regions contain nine full-length and three partial trypsin genes. A conservative estimate based on PCR analysis and genomic sequence indicated at least 22 different trypsin genes in L. salmonis, of which 18 are most similar to the previously described LsTyp1 and -2 cDNA sequences. Four of these genes are putative pseudogenes. In addition, a putative mariner like transposase gene was identified. The genomic sequences suggest that the L. salmonis trypsin genes reside within one or more gene clusters. Three different LsTryp intron exon structures were identified, and all three are different from the intron exon organisation previously reported for other S1A peptidases. This implies several intron loss and gain events in the evolution of the L. salmonis trypsin genes. (c) 2005 Elsevier B.V. All rights reserved.