Detergents for the stabilization and crystallization of membrane proteins
Detergents for the stabilization and crystallization of membrane proteins
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DOI：
10.1016/j.ymeth.2007.01.007
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发表时间：
2007-04-01
期刊：
影响因子：
4.8
通讯作者：
Prive, Gilbert G.
中科院分区：
文献类型：
--
作者：
Prive, Gilbert G.
讨论了去垢剂在膜蛋白结构研究中的应用，重点是与膜溶解、蛋白质聚集、去垢剂纯度和去垢剂定量相关的实际问题。由于其自组装特性，去垢剂是作为脂质双层模拟物的有用试剂，但因此，它们在溶液中具有复杂的特性。将溶解的膜蛋白维持在天然构象状态可能很困难，并且去污剂溶解的蛋白质的非特异性聚集是一个常见问题。经验“稳定性筛选”有助于选择对于给定系统来说最佳的去污剂和去污剂浓度。 (c) 2007 Elsevier Inc. 保留所有权利。
The use of detergents for the structural study of membrane proteins is discussed with an emphasis on practical issues relating to membrane solubilization, protein aggregation, detergent purity and detergent quantitation. Detergents are useful reagents as mimics of lipid bilayers because of their self-assembling properties, but as a result, they have complex properties in solution. It can be difficult to maintain a solubilized membrane protein in a native conformational state, and the non-specific aggregation of detergent-solubilized proteins is a common problem. Empirical "stability screens" can be helpful in choosing which detergents, and which detergent concentrations, may be optimal for a given system. (c) 2007 Elsevier Inc. All rights reserved.