Lack of glucose and hsp70 responses in haddock Melanogrammus aeglefinus (L.) subjected to handling and heat shock
Lack of glucose and hsp70 responses in haddock Melanogrammus aeglefinus (L.) subjected to handling and heat shock
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DOI：
10.1111/j.1095-8649.2007.01697.x
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发表时间：
2008-01-01
影响因子：
2
通讯作者：
Johnson, S.
中科院分区：
文献类型：
--
作者：
Afonso, L. O. B.;Hosoya, S.;Johnson, S.
将幼年黑线鳕 Melanogrammus aeglefinus（约 39 克）暴露于处理应激源（离开水 1 分钟）或热休克（从 10 摄氏度升至 15 摄氏度，持续 1 小时），并在应激前和应激后 1、3、6、12、24 和 48 小时测定血浆皮质醇、血浆葡萄糖和鳃 hsp70 水平。两种应激源后皮质醇增加的模式相似，应激后 1 小时皮质醇水平增加 25 倍，但 3 小时恢复到应激前水平 (2-5 ng ml(-1))。相反，处理和热休克均不会导致血浆葡萄糖水平升高。尽管在对照组和热休克组中都检测到了鳃 hsp70，推测是基本水平，但暴露于热休克后鳃 hsp70 水平没有显着变化。对这些典型应激源缺乏葡萄糖和 hsp70 反应与之前对大西洋鳕鱼的研究一致，并表明鳕鱼科的应激生理学不同于“典型”硬骨鱼。 (C) 2008 年加拿大国家研究委员会。
Juvenile haddock Melanogrammus aeglefinus (c. 39 g) were exposed to either a handling stressor (1 min out of water) or heat shock (increase from 10 to 15 degrees C for 1 h), and plasma cortisol, plasma glucose and gill hsp70 levels were determined before, and at 1, 3, 6, 12, 24 and 48 h post-stress. The pattern of cortisol increase was similar following both stressors, with levels increasing by 25-fold at 1 h post-stress, but returning to pre-stress levels (2-5 ng ml(-1)) by 3 h. In contrast, neither handling nor heat shock caused an increase in plasma glucose levels. Although gill hsp70 was detected, presumably constitutive levels, in both control and heat shocked groups, there were not significant changes in gill hsp70 levels after exposure to heat shock. The lack of glucose and hsp70 responses to these typical stressors is consistent with previous studies on Atlantic cod Gadus morhua, and suggests that the stress physiology of Gadidae differs from the 'typical' teleost. (C) 2008 National Research Council Canada.