Common nuclear ribosomal internal transcribed spacer sequences occur in the sibling species Ophiostoma piceae and O. quercus
Common nuclear ribosomal internal transcribed spacer sequences occur in the sibling species Ophiostoma piceae and O. quercus
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常见的核核糖体内部转录间隔区序列出现在兄弟物种云杉蛇和栎树中
DOI：
10.1017/s0953756201003422
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
C. Breuil
中科院分区：
文献类型：
--
作者：
S. Kim;C. Breuil
为了探讨利用ITS 1和ITS 2及5.8S rDNA序列作为分子标记在蛇舌菌属系统学和系统发育研究中的可行性，研究了云杉蛇舌菌（Ophiostoma piceae）和蛇舌菌（O.对来自不同地理区域的22个分离物进行了检测。PCR和序列分析结果表明，两种蛇舌藻的ITS引物ITS 1-ITS 4或ITS 5-ITS 4或ITS 1-F-ITS 4扩增产物中存在两种不同的ITS序列。令人惊讶的是，这两个ITS序列是由两个物种共享的。一个ITS区序列在O.云杉属（O. piceae）的ITS序列为OPC型，而O. Piceae被指定为OPH类型。号手术栎属以OPH型为主，OPC型次之。主要的ITS包括超过99%的扩增子在这两个物种。结果表明，共同的ITS基因库已保持在不同的水平在这些兄弟物种。
To determine the feasibility of using internal transcribed spacers (ITS1 and ITS2) and 5.8S ribosomal DNA sequences as genetic markers in molecular systematic and phylogenetic studies of Ophiostoma , the presence of multiple types of ITS in the two sibling sapstain fungi, Ophiostoma piceae and O. quercus were examined in 22 isolates from diverse geographic regions. PCR and sequence analysis revealed that two different versions of ITS sequences were present in the PCR-products amplified with the ITS primers, ITS1-ITS4 or ITS5-ITS4 or ITS1-F-ITS4, in every isolate of the two Ophiostoma species tested. Surprisingly, the two ITS sequences were shared by both species. One ITS sequence which was major in O. piceae was designated as the OPC type, and the other ITS sequence which was minor in O. piceae was designated as the OPH type. In O. quercus the OPH type was major and the OPC type was minor. The major ITS comprised more than 99% of the amplicons in both species. The results suggest that common ITS gene pools have been maintained at different levels within these sibling species.