Characterization of chromosome structures of Falconinae (Falconidae, Falconiformes, Aves) by chromosome painting and delineation of chromosome rearrangements during their differentiation
Characterization of chromosome structures of Falconinae (Falconidae, Falconiformes, Aves) by chromosome painting and delineation of chromosome rearrangements during their differentiation
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DOI：
10.1007/s10577-007-1210-6
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发表时间：
2008-03-01
影响因子：
2.6
通讯作者：
Matsuda, Yoichi
中科院分区：
文献类型：
--
作者：
Nishida, Chizuko;Ishijima, Junko;Matsuda, Yoichi
大多数鸟类的核型特征是大约 2n = 80 条染色体，包括 7-10 对大中型大型染色体，包括性染色体和许多形态上无法区分的微小染色体。隼形目隼亚科的核型与典型鸟类的核型不同，染色体数目较少，大染色体大小差异不大，小染色体数目较少。为了表征隼亚科的染色体结构并描绘该亚科中发生的染色体重排，我们使用鸡染色体 1-9 和 Z 探针以及微染色体特异性探针进行了比较染色体绘制，并对普通红隼的 18S-28S rRNA 基因和端粒 (TTAGGG) (n) 序列进行了染色体作图 (Falcotinnunculus) (2n = 52)、游隼 (Falco peregrinus) (2n = 50) 和梅林 (Falco columbarius) (2n = 40)。 F.tinnunculus 拥有最多的染色体，被认为保留了隼亚科的祖先核型； F. peregrinus 和 F. columbarius 的大染色体中可能分别发生了 1 个和 6 个中心融合。微染色体与大染色体以及微染色体之间的串联融合也经常被观察到，rRNA基因的染色体位置范围为2至7对染色体。鹰亚科的这些核型特征与鹰科的核型特征相对不同，表明剧烈的染色体重排在鹰科和鹰亚科谱系中独立发生。
Karyotypes of most bird species are characterized by around 2n = 80 chromosomes, comprising 7-10 pairs of large- and medium-sized macrochromosomes including sex chromosomes and numerous morphologically indistinguishable microchromosomes. The Falconinae of the Falconiformes has a different karyotype from the typical avian karyotype in low chromosome numbers, little size difference between macrochromosomes and a smaller number of microchromosomes. To characterize chromosome structures of Falconinae and to delineate the chromosome rearrangements that occurred in this subfamily, we conducted comparative chromosome painting with chicken chromosomes 1-9 and Z probes and microchromosome-specific probes, and chromosome mapping of the 18S-28S rRNA genes and telomeric (TTAGGG) (n) sequences for common kestrel (Falco tinnunculus) (2n = 52), peregrine falcon (Falco peregrinus) (2n = 50) and merlin (Falco columbarius) (2n = 40). F. tinnunculus had the highest number of chromosomes and was considered to retain the ancestral karyotype of Falconinae; one and six centric fusions might have occurred in macrochromosomes of F. peregrinus and F. columbarius, respectively. Tandem fusions of microchromosomes to macrochromosomes and between microchromosomes were also frequently observed, and chromosomal locations of the rRNA genes ranged from two to seven pairs of chromosomes. These karyotypic features of Falconinae were relatively different from those of Accipitridae, indicating that the drastic chromosome rearrangements occurred independently in the lineages of Accipitridae and Falconinae.