Distribution and number of spermatozoa in the oviduct of the golden hamster after natural mating and artificial insemination.
Distribution and number of spermatozoa in the oviduct of the golden hamster after natural mating and artificial insemination.
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DOI：
10.1095/biolreprod37.1.225
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发表时间：
1987-08
影响因子：
3.6
通讯作者：
T. T. Smith-T.;F. Koyanagi;R. Yanagimachi
中科院分区：
文献类型：
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作者：
T. T. Smith-T.;F. Koyanagi;R. Yanagimachi
一组雌性仓鼠在排卵前几个小时或排卵期间与已被证实具有生育能力的雄性仓鼠交配。另一组雌性在排卵前几个小时进行了人工授精。在交配或人工授精开始后的不同时间处死雌性，固定并连续切开输卵管，逐个计数精子在输卵管中的位置。无论授精的类型或时间如何，绝大多数进入输卵管的精子都留在峡部的下部(管壁内和尾部峡部)，而不会上升到壶腹。输卵管的下段，特别是尾部峡部，似乎扮演着“筛子”和/或“精子储存库”的角色。在排卵前交配或人工授精的雌性，几乎没有精子到达头峡部或壶腹，直到排卵开始。虽然有少数精子在交配开始后1h到达壶腹，但它们是例外而不是规则。当雌性在排卵期间交配时，精子在上升到壶腹之前至少在尾部峡部停留约3小时。人工授精后进入输卵管的精子数量比自然交配的动物要少得多，但这个低数量显然足以确保完全受精。
A group of female hamsters was mated with males of proven fertility either several hours before or during ovulation. Another group of females was artificially inseminated several hours before ovulation. Females were killed at various times after the onset of mating or artificial insemination, oviducts were fixed and sectioned serially, and spermatozoa were counted individually as to their location in the oviduct. Regardless of the type or time of insemination, the vast majority of spermatozoa that entered the oviduct remained in the lower segments of the isthmus (the intramural and caudal isthmus) without ascending to the ampulla. The lower segments of the oviduct, particularly the caudal isthmus, appeared to be acting as a "sieve" and/or "sperm reservoir." In females mated or artificially inseminated prior to ovulation, virtually no spermatozoa reached the cephalic isthmus or ampulla until the commencement of ovulation. Although a few spermatozoa reached the ampulla by 1 h after the onset of mating, they were the exception rather than the rule. When females were mated during ovulation, spermatozoa spent a minimum of about 3 h in the caudal isthmus before ascending to the ampulla. The number of spermatozoa that entered the oviduct after artificial insemination was considerably lower than in naturally mated animals, but this low number was apparently large enough to ensure complete fertilization.