CHANGING THE RNA-POLYMERASE SPECIFICITY OF U-SNRNA GENE PROMOTERS
CHANGING THE RNA-POLYMERASE SPECIFICITY OF U-SNRNA GENE PROMOTERS
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DOI：
10.1016/0092-8674(88)90029-3
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发表时间：
1988-11-04
期刊：
影响因子：
64.5
通讯作者：
KROL, A
中科院分区：
文献类型：
--
作者：
MATTAJ, IW;DATHAN, NA;KROL, A
热带爪蟾U6基因的启动子可以由RNA聚合酶II和III转录。两个不同的元件，TATA样序列和转录起始区域，仅需要通过RNA聚合酶III转录，而进一步的共同元件是需要通过两种聚合酶转录。基于异常严格的要求嘌呤在正常位置的聚合酶III转录起始和在该区域的突变体的属性，我们建议，RNA聚合酶III本身可以识别的转录起始位点，从而直接参与有效的启动子选择。我们已经使用U6启动子结构上获得的信息来制造RNA聚合酶II特异性的U6启动子，并将非洲爪蟾U2基因启动子特异性从RNA聚合酶II改变为RNA聚合酶III。
The promoter of a Xenopus tropicalis U6 gene can be transcribed by both RNA polymerases II and III. Two distinct elements, a TATA-like sequence and the region of transcription initiation, are only required for transcription by RNA polymerase III, while further common elements are required for transcription by both polymerases. Based on the unusually stringent requirement for a purine at the normal position of polymerase III transcription initiation and on the properties of mutants in this region, we suggest that RNA polymerase III itself may recognize the site of transcription initiation and thus be directly involved in efficient promoter selection. We have used the information obtained on U6 promoter structure to manufacture a U6 promoter that is RNA polymerase II-specific and to change the Xenopus U2 gene promoter specificity from RNA polymerase II to RNA polymerase III.