Two essential DNA polymerases at the bacterial replication fork
Two essential DNA polymerases at the bacterial replication fork
复制标题
DOI：
10.1126/science.1066351
复制
发表时间：
2001-11-23
期刊：
影响因子：
56.9
通讯作者：
Ehrlich, SD
中科院分区：
文献类型：
--
作者：
Dervyn, E;Suski, C;Ehrlich, SD
细菌中的DNA复制是由一个多蛋白复合体进行的，该复合体被认为只包含一种必需的DNA聚合酶，由大肠杆菌中的dhaE基因和枯草芽孢杆菌中的polC基因指定。枯草芽孢杆菌基因组分析发现了另一个DNA聚合酶基因dnaE(BS)，该基因与dnaE同源。我们发现，在枯草芽孢杆菌中，dnaE（BS）对细胞活力和DNA复制的延伸步骤至关重要，就像polC一样，我们得出结论，在枯草芽孢杆菌的复制叉上有两种不同的必要DNA聚合酶，正如之前在真核生物中观察到的那样。dnaE（BS）似乎参与了标记DNA链的合成，并与复制工厂有关，这表明在枯草芽孢杆菌和许多其他含有polC和dnaE基因的细菌中，有两种不同的聚合酶进行这两条DNA链的合成。
DNA replication in bacteria is carried out by a multiprotein complex, which is thought to contain only one essential DNA polymerase, specified by the dhaE gene in Escherichia coli and the polC gene in Bacillus subtilis. Bacillus subtilis genome analysis has revealed another DNA polymerase gene, dnaE(BS), which is homologous to dnaE. We show that, in B. subtilis, dnaE(BS) is essential for cell viability and for the elongation step of DNA replication, as is polC, and we conclude that there are two different essential DNA polymerases at the replication fork of B. subtilis, as was previously observed in eukaryotes. dnaE(BS) appears to be involved in the synthesis of the tagging DNA strand and to be associated with the replication factory, which suggests that two different polymerases carry out synthesis of the two DNA strands in B. subtilis and in many other bacteria that contain both polC and dnaE genes.