Determination of catechins in human urine subsequent to tea ingestion by high-performance liquid chromatography with electrochemical detection.
Determination of catechins in human urine subsequent to tea ingestion by high-performance liquid chromatography with electrochemical detection.
复制标题
采用高效液相色谱法和电化学检测法测定摄入茶后人尿中的儿茶素。
作者：
Bin Yang;K. Arai;F. Kusu
采用高效液相色谱-电化学检测法，以乙腈：0.1 M磷酸盐缓冲液（pH2.5）（15：85，v/v）为移动的流动相，ODS柱为分离柱。在0.6V（相对于Ag/AgCl）下检测八种儿茶素，即没食子儿茶素（GC）、表没食子儿茶素（EGC）、儿茶素（C）、表儿茶素（EC）、表没食子儿茶素没食子酸酯（EGCg）、没食子儿茶素没食子酸酯（GCg）、表儿茶素没食子酸酯（ECg）和儿茶素没食子酸酯（Cg）。对于0.5-250 pmol的每种儿茶素，注意到电流和量之间的良好线性关系，在每种情况下的相关系数为0.999。任何一种的检测限为0.5 pmol（信噪比，S/N = 3）。在摄入340 ml罐装绿色茶后，在尿液样品中测定GC、EGC、C和EC，主要以结合形式存在。共轭儿茶素水解酶使用硫酸酯酶和β-葡萄糖醛酸酶。上述四种儿茶素的时程变化均在饮茶后1-3 h达到最大值。（+）、（-）-EC和（+）、（-）-C在罐装茶中均有检出。
The title determination was conducted by HPLC with electrochemical detection using an ODS column and a mobile phase of acetonitrile: 0.1 M phosphate buffer (pH 2.5) (15:85, v/v). The eight catechins, gallocatechin (GC), epigallocatechin (EGC), catechin (C), epicatechin (EC), epigallocatechin gallate (EGCg), gallocatechin gallate (GCg), epicatechin gallate (ECg), and catechin gallate (Cg), were detected at 0.6 V vs Ag/AgCl. Good linear relationships between current and amount were noted for 0.5-250 pmol of each catechin, with a correlation coefficient of 0.999 in each case. The detection limit for any one was 0.5 pmol (signal to noise ratio, S/N = 3). After the ingestion of 340 ml canned green tea, GC, EGC, C, and EC, mostly in conjugated form, were determined in urine samples. Conjugated catechins were hydrolyzed by enzymes using sulfatase and beta-glucuronidase. The time courses of the above four catechins showed a maxima at 1-3 h after tea ingestion. (+), (-)-EC and (+), (-)-C were present in canned tea.
DOI：
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发表时间：
1995-06
期刊：
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology
影响因子：
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作者：
Mao-jung Lee;Zhi-Yuan Wang;He Li;Laishun Chen;Yang Sun;Steve Gobbo;D. Balentine;Chung S. Yane
通讯作者：
Mao-jung Lee;Zhi-Yuan Wang;He Li;Laishun Chen;Yang Sun;Steve Gobbo;D. Balentine;Chung S. Yane