Electrochemical impedance spectroscopy detection of lysozyme based on electrodeposited gold nanoparticles
Electrochemical impedance spectroscopy detection of lysozyme based on electrodeposited gold nanoparticles
复制标题
基于电沉积金纳米粒子的电化学阻抗谱检测溶菌酶
DOI：
10.1016/j.talanta.2010.11.042
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发表时间：
2011-02-15
期刊：
影响因子：
6.1
通讯作者：
Mu, Xiaojiao
中科院分区：
文献类型：
--
作者：
Chen, Zhengbo;Li, Lidong;Mu, Xiaojiao
以抗溶菌酶适体为分子识别元件，研制了一种简单、高灵敏、无标记的电化学阻抗谱（EIS）适体传感器，用于检测溶菌酶。通过利用电沉积到金电极表面上的金纳米颗粒（AuNPs）作为用于固定适体的平台，实现了灵敏度的改善。为了定量溶菌酶的量，使用[Fe（CN）（6）]（3-/4-）探针的氧化还原对监测适体传感器的界面电子转移电阻（R-et）的变化。R-et随溶菌酶浓度的增加而增加。R-et对溶菌酶浓度对数的曲线在0.1 pM至500 pM范围内呈线性，检测限为0.01 pM。适体传感器也表现出良好的选择性，而不受其他蛋白质的存在下的溶菌酶。(C)2010爱思唯尔有限公司版权所有。
A simple, highly sensitive, and label-free electrochemical impedance spectroscopy (EIS) aptasensor based on an anti-lysozyme-aptamer as a molecular recognition element, was developed for the detection of lysozyme. Improvement in sensitivity was achieved by utilizing gold nanoparticles (AuNPs), which were electrodeposited onto the surface of a gold electrode, as a platform for immobilization of the aptamer. To quantify the amount of lysozyme, changes in the interfacial electron transfer resistance (R-et) of the aptasensor were monitored using the redox couple of an [Fe(CN)(6)](3-/4-) probe. The R-et increased with lysozyme concentration. The plot of R-et against the logarithm of lysozyme concentration is linear over the range from 0.1 pM to 500 pM with a detection limit of 0.01 pM. The aptasensor also showed good selectivity for lysozyme without being affected by the presence of other proteins. (C) 2010 Elsevier B.V. All rights reserved.