Role of CD4+ T Helper Cells in the Development of BAC-Induced Dry Eye Syndrome in Mice.
Role of CD4+ T Helper Cells in the Development of BAC-Induced Dry Eye Syndrome in Mice.
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CD4 T 辅助细胞在 BAC 诱导的小鼠干眼综合征发生中的作用
DOI：
10.1167/iovs.62.1.25
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发表时间：
2021-01-04
影响因子：
4.4
通讯作者：
Liu Z
中科院分区：
文献类型：
--
作者：
Ouyang W;Wu Y;Lin X;Wang S;Yang Y;Tang L;Liu Z;Wu J;Huang C;Zhou Y;Zhang X;Hu J;Liu Z
目的探讨CD4+ T辅助细胞在苯扎氯铵（benzalkonium chloride， BAC）致C57BL/6小鼠眼表疾病中的作用。方法0.075% BAC外用于C57BL/6小鼠，每日2次，连续7 d；pbs处理和未处理的小鼠作为对照。将从bac处理小鼠或pbs处理小鼠中分离的CD4+ T细胞过继转移到裸鼠中，以鉴定CD4+ T细胞的作用，并将未处理的裸鼠作为对照。分别对两种模型进行俄勒冈绿葡聚糖染色、PAS染色和酚红棉试验。分别采用定量RT-PCR和ELISA检测T-bet、IFN-γ、rorr γt和IL-17的基因和蛋白水平。采用双免疫荧光染色和流式细胞术检测CD4+ T细胞的活化和亚群。结果BAC可诱导C57BL/6小鼠CD4+CD69+、CD4+IFN-γ+和CD4+IL-17+细胞升高。与对照小鼠相比，bac处理小鼠的IFN-γ、IL-17、Th1、Th17以及转录因子T-bet和RORγt均升高。此外，BAC还会引起眼表面损伤，包括角膜屏障功能障碍、杯状细胞丢失和泪液分泌减少。有趣的是，从BAC处理的小鼠中分离的CD4+ T细胞过继转移到裸鼠中，导致眼表表现与直接局部BAC处理C57BL/6小鼠相似，包括CD4+ T细胞增加，IFN-γ， IL-17和眼表疾病。结论局部应用BAC部分通过CD4+ T细胞介导的炎症反应诱导眼表干眼症。
Purpose To evaluate the role of CD4+ T helper cells in benzalkonium chloride (BAC)-induced ocular surface disorder in C57BL/6 mice. Methods Topical 0.075% BAC was applied twice daily in C57BL/6 mice for 7 consecutive days; PBS-treated and untreated mice served as controls. Adoptive transfer of CD4+ T cells isolated from the BAC-treated mice or PBS-treated mice into nude mice was conducted to identify the roles of CD4+ T cells, with untreated nude mice as controls. Oregon green dextran staining, PAS staining, and the phenol red cotton test were carried out in these two models. The gene and protein levels of T-bet, IFN-γ, RORγt, and IL-17 were detected by quantitative RT-PCR and ELISA, respectively. The activation and subsets of CD4+ T cells were identified by double immunofluorescent staining and flow cytometry. Results An increase in CD4+CD69+, CD4+IFN-γ+, and CD4+IL-17+ cells was induced by BAC in C57BL/6 mice. IFN-γ, IL-17, Th1, Th17, and the transcription factors T-bet and RORγt were increased in BAC-treated mice compared with control mice. In addition, ocular surface damage, including corneal barrier dysfunction, goblet cell loss, and decreased tear production, was induced by BAC. Interestingly, adoptive transfer of CD4+ T cells isolated from BAC-treated mice into nude mice resulted in ocular surface manifestations similar to those of direct topical BAC treatment of C57BL/6 mice, including increased CD4+ T cells, IFN-γ, IL-17, and ocular surface disorders. Conclusions Topical application of BAC induced a dry-eye-like ocular surface disorder partly through the CD4+ T cell-mediated inflammatory response.
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局部应用防腐剂苯扎氯铵诱导兔干眼模型的稳定性研究
影响因子：
3.7
作者：
Li C;Song Y;Luan S;Wan P;Li N;Tang J;Han Y;Xiong C;Wang Z
通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
Pflugfelder SC
DOI：
10.1016/j.clim.2018.04.009
发表时间：
2018-07
期刊：
Clinical immunology (Orlando, Fla.)
影响因子：
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作者：
Voigt A;Bohn K;Sukumaran S;Stewart CM;Bhattacharya I;Nguyen CQ
通讯作者：
Nguyen CQ
影响因子：
3.4
作者：
Soriano-Romani, Laura;Garcia-Posadas, Laura;Diebold, Yolanda
通讯作者：
Diebold, Yolanda