Alzheimer's disease diagnosis by detecting exogenous fluorescent signal of ligand bound to Beta amyloid in the lens of human eye: an exploratory study.
Alzheimer's disease diagnosis by detecting exogenous fluorescent signal of ligand bound to Beta amyloid in the lens of human eye: an exploratory study.
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DOI：
10.3389/fneur.2013.00062
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发表时间：
2013
影响因子：
3.4
通讯作者：
Hartung PD
中科院分区：
文献类型：
--
作者：
Kerbage C;Sadowsky CH;Jennings D;Cagle GD;Hartung PD
我们报告了一项临床探索性人体试验的结果，该试验涉及10名参与者，使用荧光配体和激光扫描设备SAPPHIRE系统的组合，作为诊断可能的阿尔茨海默病（AD）的辅助手段。据我们所知，这是第一次在人体透镜的体内使用这种技术。除了安全性评估外，临床试验的主要目的是评价系统的有效性。通过检测人透镜的核上（SN）区域中配体结合的β淀粉样蛋白的特异性荧光特征，获得了AD患者和对照组之间的双重区分因子。来自我们的研究的数据表明，SN的较深区域提供了来自对照和AD患者的配体结合荧光信号的最高测量。此外，我们目前的临床前研究，进行调查的结合亲和力的配体β淀粉样蛋白和评估的配体在兔眼的药代动力学。进一步的研究正在进行中，涉及更大的人群，用于该方法的统计学评价。
We report results of a clinical exploratory human trial involving 10 participants using a combination of a fluorescent ligand and a laser scanning device, SAPPHIRE System, as an aid in the diagnosis of Probable Alzheimer’s disease (AD). To the best of our knowledge, this is the first time that such a technique has been used in vivo of a human lens. The primary goal of the clinical trial, in addition to safety assessment, was to evaluate efficacy of the system. By detecting specific fluorescent signature of ligand bound beta amyloid in the supranucleus (SN) region of the human lens, a twofold differentiation factor between AD patients and Control groups is achieved. Data from our studies indicates that deeper regions of the SN provide the highest measures of ligand bound fluorescence signal from both controls and patients with AD. In addition, we present preclinical studies that were performed to investigate the binding affinity of the ligand to beta amyloid and evaluate the pharmacokinetics of the ligand in rabbit eyes. Further studies are underway involving a larger population for statistical evaluation of the method.
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影响因子：
5.7
作者：
Koronyo-Hamaoui, Maya;Koronyo, Yosef;Ljubimov, Alexander V.;Miller, Carol A.;Ko, MinHee K.;Black, Keith L.;Schwartz, Michal;Farkas, Daniel L.
通讯作者：
Farkas, Daniel L.
DOI：
10.1016/j.jalz.2011.03.005
发表时间：
2011-05
期刊：
Alzheimer's & dementia : the journal of the Alzheimer's Association
影响因子：
--
作者：
McKhann GM;Knopman DS;Chertkow H;Hyman BT;Jack CR Jr;Kawas CH;Klunk WE;Koroshetz WJ;Manly JJ;Mayeux R;Mohs RC;Morris JC;Rossor MN;Scheltens P;Carrillo MC;Thies B;Weintraub S;Phelps CH
通讯作者：
Phelps CH
DOI：
10.1016/s1474-4422(12)70228-4
发表时间：
2012-12
期刊：
The Lancet. Neurology
影响因子：
--
作者：
Reiman EM;Quiroz YT;Fleisher AS;Chen K;Velez-Pardo C;Jimenez-Del-Rio M;Fagan AM;Shah AR;Alvarez S;Arbelaez A;Giraldo M;Acosta-Baena N;Sperling RA;Dickerson B;Stern CE;Tirado V;Munoz C;Reiman RA;Huentelman MJ;Alexander GE;Langbaum JB;Kosik KS;Tariot PN;Lopera F
通讯作者：
Lopera F
DOI：
10.1111/j.1532-5415.1998.tb04543.x
发表时间：
1998-10-01
影响因子：
6.3
作者：
Esteban-Santillan, C;Praditsuwan, R;Geldmacher, DS
通讯作者：
Geldmacher, DS
影响因子：
2
作者：
Frederikse, PH
通讯作者：
Frederikse, PH