Knockdown of GmVQ58 encoding a VQ motif-containing protein enhances soybean resistance to the common cutworm (Spodoptera litura Fabricius)
Knockdown of GmVQ58 encoding a VQ motif-containing protein enhances soybean resistance to the common cutworm (Spodoptera litura Fabricius)
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敲低编码含 VQ 基序的蛋白质的 GmVQ58 可增强大豆对斜纹夜蛾 (Spodoptera litura Fabricius) 的抗性
DOI：
10.1093/jxb/eraa095
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发表时间：
2020-05-30
影响因子：
6.9
通讯作者：
Yu, Deyue
中科院分区：
文献类型：
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作者：
Li, Xiao;Qin, Rui;Yu, Deyue
植物进化出复杂的防御机制来抵御昆虫的攻击。植物内源抗虫基因的鉴定对于了解植物-植食性昆虫互作和提高作物抗虫性具有重要意义。大豆（Glycine max（L.）梅尔）斜纹夜蛾（Spodoptera litura Fabricius）是重要的农作物，也是常见的地老虎。在这项研究中，根据我们的转录组数据，基因GmVQ 58，编码一个FxxxVQxxTG（VQ）基序的蛋白质，克隆和表征。该基因在叶片和根中表达量最高，在CCW攻击后表达显著上调。GmVQ 58的组成型表达挽救了拟南芥突变体对CCW的敏感性，并且GmVQ 58在大豆毛状根中的干扰增强了对CCW的抗性。此外，GmVQ 58定位于细胞核，并与转录因子GmWRKY 32物理相互作用。两个防御相关基因GmN：IFR和GmVSP β的表达在GmVQ 58-RNAi株系中上调。此外，GmVQ 58的启动子区域可能在驯化过程中被选择，导致栽培大豆相对于野生大豆的不同表达模式。这些结果表明沉默GmVQ 58赋予大豆对CCW的抗性。
Plants have evolved complex defense mechanisms to withstand insect attack. Identification of plant endogenous insect resistance genes is of great significance for understanding plant-herbivore interactions and improving crop insect resistance. Soybean (Glycine max (L.) Merr.) is an important crop that is often attacked by the common cutworm (CCW) (Spodoptera litura Fabricius). In this study, based on our transcriptomic data, the gene GmVQ58, encoding a FxxxVQxxTG (VQ) motif-containing protein, was cloned and characterized. This gene showed the highest expression in the leaves and roots and was up-regulated significantly after CCW attack. Constitutive expression of GmVQ58 rescued the susceptibility of an Arabidopsis mutant to CCW, and interference of GmVQ58 in soybean hairy roots enhanced the resistance to CCW. Furthermore, GmVQ58 was localized to the nucleus and physically interacted with the transcription factor GmWRKY32. The expression of two defense-related genes, GmN:IFR and GmVSP beta, was up-regulated in GmVQ58-RNAi lines. Additionally, the promoter region of GmVQ58 was likely selected during domestication, resulting in different expression patterns in cultivated soybeans relative to wild soybeans. These results suggest that silencing GmVQ58 confers soybean resistance to CCW.