Detailed structure of Pneumocystis carinii chromosome ends.
Detailed structure of Pneumocystis carinii chromosome ends.
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卡氏肺囊虫染色体末端的详细结构。
DOI：
10.1111/j.1550-7408.2001.tb00477.x
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Stringer,JR
中科院分区：
文献类型：
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作者：
Keely,SP;Wakefield,AE;Cushion,MT;Smulian,AG;Hall,N;Barrell,BG;Stringer,JR
2P内包含的基因阵列序列。测定了染色体3G5和1b2。3G5中的DNA始于4个假定基因(开放阅读框架(ORF)1、NMP、Map和ORF2)，它们似乎是独一无二的，因为它们杂交到相同的染色体上，这是大约的。315kb大小。ORF2之后是一个1kb的区域，与主要表面糖蛋白(MSG)基因的3‘端匹配。1kb的味精样区标志着3G5插入的重复区域的开始。在这之后是一组包含3个重复基因家族成员的基因。基因簇的末端由亚端粒DNA标记，随后是一小段端粒。粘粒11H12和1b2携带的基因组片段重叠。重复的基因簇以味精相关(MSR)基因开始，紧随其后的是蛋白酶1(PRT1)基因，紧随其后的是MSR和味精，但随后还有第二个PRT1基因。就像3G5携带一个不完整的味精基因一样，1b2携带两个DNA片段，似乎是MSR或MSG基因的片段。在第一个MSR基因之后是一个类似于味精基因5‘端的序列。3G5和1b2粘粒序列的登录号分别为AL592382和AL592263。
The sequence of gene arrays contained within 2P. cariniicosmids, 3G5 and 1B2, were determined. The DNA in 3G5 begins with 4 presumptive genes (open reading frame (ORF) 1,Nmp, Map, and ORF2) that appeared to be unique because they hybridized to the same chromosome which was approx. 315 kb in size. Following ORF2 is a 1 kb region that matches the 3′ ends of major surface glycoprotein (MSG) genes. The 1 kb MSG-like region marks the beginning of the repeated region of the 3G5 insert. After this comes a cluster of genes containing members of the 3 repeated gene families. The end of the gene cluster is marked by subtelomeric DNA which is followed by a short stretch of telomere. The genome segment carried by cosmids 11H12 and 1B2 overlaps. The repeated gene cluster begins with an MSG-related (MSR) gene, followed by a protease 1 (PRT1) gene, which is followed by an MSR and an MSG, but then there is a 2nd PRT1 gene. Like 3G5, which carried one incomplete MSG gene, 1B2 carries 2 segments of DNA that appear to be segments of either MSR or MSG genes. Following the first MSR gene is a sequence resembling the 5′ end of an MSG gene. The accession numbers for the cosmid sequences are AL592382 and AL592263 for cosmids 3G5 and 1B2, respectively.