3-D Printed Adjustable Microelectrode Arrays for Electrochemical Sensing and Biosensing.
3-D Printed Adjustable Microelectrode Arrays for Electrochemical Sensing and Biosensing.
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DOI：
10.1016/j.snb.2016.02.113
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发表时间：
2016-07
期刊：
影响因子：
--
通讯作者：
Lin Y
中科院分区：
文献类型：
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作者：
Yang H;Rahman T;Du D;Panat R;Lin Y
印刷电子学已经成为一种重要的制造技术，它克服了传统光刻的几个缺点，并为各种传感器应用提供定制的快速原型。在这项工作中，银微电极阵列（MEA）与三个不同的电极间距的气溶胶喷射技术使用3-D打印。以约15 μm的长度尺度印刷微电极，其中电极之间的间距分别精确控制为迹线宽度的约2倍（30 μm，MEA 30）、6.6倍（100 μm，MEA 100）和12倍（180 μm，MEA 180）。选择过氧化氢和葡萄糖作为模型分析物，以证明MEA用于传感器应用的性能。电极被示出为对于某些浓度范围以与过氧化氢的浓度成比例的还原电流还原过氧化氢。此外，三种电极配置的电流灵敏度显示出随着微电极间距的增加而降低（MEA 30：MEA 100：MEA 180的灵敏度为3.7：2.8：1的比率），证明了用于此类应用的最佳MEA几何形状。还表征了不同电极配置的噪声，并且显示出从MEA 30到MEA 100和MEA 180电极的显著降低。进一步地，示出了响应电流与MEA 100和MEA 180电极面积成比例，但不与MEA 30电极的面积成比例（MEA 30：MEA 100：MEA 180的电流密度为0.25：1：1），表明MEA 30电极遭受来自相邻电极的扩散重叠。因此，这项工作建立了我们的几何形状的微电极间距的下限。MEA的最低检测限计算（S/N = 3）为0.45 μM。将葡萄糖氧化酶固定在MEA 100微电极上，以展示葡萄糖生物传感器的应用。该传感器的灵敏度为1.73 μAmM-1，检出限（S/N = 3）为1.7 μM。MEA的电化学响应特性与现有模型的预测一致。目前的工作开辟了增材制造作为低成本定制形状MEA结构的制造技术的可能性，该MEA结构可用作广泛传感器应用的电化学平台。
Printed Electronics has emerged as an important fabrication technique that overcomes several shortcomings of conventional lithography and provides custom rapid prototyping for various sensor applications. In this work, silver microelectrode arrays (MEA) with three different electrode spacing were fabricated using 3-D printing by the aerosol jet technology. The microelectrodes were printed at a length scale of about 15 μm, with the space between the electrodes accurately controlled to about 2 times (30 μm, MEA30), 6.6 times (100 μm, MEA100) and 12 times (180 μm, MEA180) the trace width, respectively. Hydrogen peroxide and glucose were chosen as model analytes to demonstrate the performance of the MEA for sensor applications. The electrodes are shown to reduce hydrogen peroxide with a reduction current proportional to the concentration of hydrogen peroxide for certain concentration ranges. Further, the sensitivity of the current for the three electrode configurations was shown to decrease with an increase in the microelectrode spacing (sensitivity of MEA30: MEA100: MEA180 was in the ratio of 3.7: 2.8: 1), demonstrating optimal MEA geometry for such applications. The noise of the different electrode configurations is also characterized and shows a dramatic reduction from MEA30 to MEA100 and MEA180 electrodes. Further, it is shown that the response current is proportional to MEA100 and MEA180 electrode areas, but not for the area of MEA30 electrode (the current density of MEA30 : MEA100 : MEA180 is 0.25 : 1 : 1), indicating that the MEA30 electrodes suffer from diffusion overlap from neighboring electrodes. The work thus establishes the lower limit of microelectrode spacing for our geometry. The lowest detection limit of the MEAs was calculated (with S/N = 3) to be 0.45 μM. Glucose oxidase was immobilized on MEA100 microelectrodes to demonstrate a glucose biosensor application. The sensitivity of glucose biosensor was 1.73 μAmM-1 and the calculated value of detection limit (S/N = 3) was 1.7 μM. The electrochemical response characteristics of the MEAs were in agreement with the predictions of existing models. The current work opens up the possibility of additive manufacturing as a fabrication technique for low cost custom-shaped MEA structures that can be used as electrochemical platforms for a wide range of sensor applications.