Cocaine increases extraneuronal levels of aspartate and glutamate in the nucleus accumbens.
Cocaine increases extraneuronal levels of aspartate and glutamate in the nucleus accumbens.
复制标题
可卡因会增加伏隔核中天冬氨酸和谷氨酸的神经元外水平。
DOI：
10.1016/0006-8993(95)00383-2
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发表时间：
1995
期刊：
影响因子：
2.9
通讯作者：
Robinson,SE
中科院分区：
文献类型：
--
作者：
Smith,JA;Mo,Q;Guo,H;Kunko,PM;Robinson,SE
脑内微透析被用来评估可卡因盐酸对细胞外浓度的兴奋性氨基酸天冬氨酸和谷氨酸在清醒的，自由活动的大鼠延髓核。在初始平衡期后，腹膜内注射可卡因（7.5、15或30 mg/kg）或盐水，再收集样品2小时。最高剂量的可卡因（30 mg/kg，i. p.）导致谷氨酸水平比基线增加4倍，天冬氨酸水平比基线增加18倍。为了验证细胞外天冬氨酸和谷氨酸的来源是神经元，使用无Ca 2+微透析缓冲液和含有10 μM河豚毒素的缓冲液进行了额外的实验。局部灌注无钙缓冲液减少了细胞外天冬氨酸和谷氨酸的增加，在大鼠注射30毫克/公斤可卡因。河豚毒素显着降低可卡因引起的兴奋性氨基酸的增加，但不是行为反应。
Intracerebral microdialysis was used to assess the effects of cocaine-HCl on extracellular concentrations of the excitatory amino acids aspartate and glutamate in the nucleus accumbens of awake, freely moving rats. After an initial equilibration period, cocaine (7.5, 15 or 30 mg/kg) or saline was injected i.p., and samples were collected for an additional 2 h. The highest dose of cocaine (30 mg/kg, i.p.) caused a 4-fold increase in glutamate levels and an 18-fold increase in aspartate levels over baseline. To verify that the source of the extracellular aspartate and glutamate was neuronal, additional experiments were conducted using Ca2+-free microdialysis buffer, and buffer containing 10 μM tetrodotoxin. Local perfusion with Ca2+-free buffer reduced the increase of extracellular aspartate and glutamate in rats injected with 30 mg/kg cocaine. Tetrodotoxin significantly decreased the cocaine-induced increase in excitatory amino acids, but not the behavioral response.