PATHOGENICITY TEST FOR LISTERIA-MONOCYTOGENES USING IMMUNOCOMPROMISED MICE
PATHOGENICITY TEST FOR LISTERIA-MONOCYTOGENES USING IMMUNOCOMPROMISED MICE
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DOI：
10.1128/jcm.25.11.2085-2089.1987
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发表时间：
1987-11-01
影响因子：
9.4
通讯作者：
LOVETT, J
中科院分区：
文献类型：
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作者：
STELMA, GN;REYES, AL;LOVETT, J
李斯特菌分离株的致死率是用正常成年小鼠和因每公斤 20 毫克角叉菜胶处理而免疫功能低下的小鼠测定的。免疫受损小鼠中致病分离株的平均 50% 致死剂量 (LD50) 显着低于未治疗小鼠 (α = 0.05)，平均减少 5.8 log10 单位。相比之下，免疫受损小鼠中非致病性分离株的平均 LD50 较低，平均仅为 0.4 log10 单位，差异并不显着（α = 0.05）。当使用免疫功能低下的小鼠时，致病性单核细胞增生李斯特菌分离株的 LD50 低于非致病性李斯特菌和 L. seeligeri 分离株的 .gtoreq。 6 log10 单位，低于 .gtoreq 的非致病性 L. ivanovii 分离株。 4 log10 单位。致病性单核细胞增多性李斯特菌分离株可通过其以每只小鼠约104 CFU的剂量在3天内引起免疫受损小鼠死亡的能力来与非致病性分离株区分开。使用铁超载小鼠的替代方法未能有效区分致病性李斯特菌分离株。
The lethality of Listeria isolates was determined with normal adult mice and mice that were immunocompromised by treatment with 20 mg of carrageenan per kg. The mean 50% lethal doses (LD50s) of the pathogenic isolates were significantly lower (.alpha. = 0.05) in the immunocompromised mice than in the untreated mice, with an average reduction of 5.8 log10 units. In contrast, the mean LD50s of the nonpathogenic isolates were lower in the immunocompromised mice by an averge of only 0.4 log10 unit, a difference that was not significant (.alpha. = 0.05). When immunocompromised mice were used, the LD50s of pathogenic Listeria monocytogenes isolates were lower than those of nonpathogenic L. innocua and L. seeligeri isolates by .gtoreq. 6 log10 units and lower than those of nonpathogenic L. ivanovii isolates by .gtoreq. 4 log10 units. Pathogenic L. monocytogenes isolates could be distinguished from nonpathogenic isolates by their ability to cause deaths in immunocompromised mice in 3 days at a dose of .apprx.104 CFU per mouse. An alternative procedure using iron-overloaded mice failed to effectively differentiate pathogenic Listeria isolates.