Rapid, low-technology MIC determination with clinical Mycobacterium tuberculosis isolates by using the microplate Alamar Blue assay
Rapid, low-technology MIC determination with clinical Mycobacterium tuberculosis isolates by using the microplate Alamar Blue assay
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DOI：
10.1128/jcm.36.2.362-366.1998
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发表时间：
1998-02-01
影响因子：
9.4
通讯作者：
Gilman, RH
中科院分区：
文献类型：
--
作者：
Franzblau, SG;Witzig, RS;Gilman, RH
采用比色法测定异烟肼（INH）、利福平（rifampin）、链霉素（SM）和乙胺丁醇（EMB）对34株秘鲁结核分枝杆菌的最低抑菌浓度（MICs）（包括泛敏感和多重耐药菌株）和H（37）Rv菌株，所有分离株的结果在8天内可获得，在16株INH敏感分离株中的3株、31株EMB敏感分离株中的5株和4株SM耐药分离株中的2株的初始检测中观察到不一致结果（通过BACTEC 460系统），MABA法和BACTEC 460法测定的MIC结果的一致性分别为：初始结果87.9%和93.6%。在对17个样品中的12个进行重新测试后，结果不一致，MABA终点的解释随着技术经验而改进。MABA是一种简单、快速、低成本、适当的技术，不需要昂贵的仪器，并且使用无毒、温度稳定的试剂。
A colorimetric, microplate-based Alamar Blue assay (MABA) method was used to determine the MICs of isoniazid (INH), rifampin, streptomycin (SM), and ethambutol (EMB) for 34 Peruvian Mycobacterium tuberculosis isolates (including both pansensitive and multidrug-resistant strains) and the H(37)Rv strain by using bacterial suspensions prepared directly from solid media, Results for all isolates were available within 8 days, Discordant results were observed on initial tests for 3 of 16 INH-susceptible isolates, 5 of 31 EMB-susceptible isolates, and 2 of 4 SM-resistant isolates (by the BACTEC 460 system), The overall agreements between the MICs obtained by MABA and the results obtained with the BACTEC 460 system were 87.9% for initial results and 93.6% after retesting 12 of 17 samples with discrepant results, Interpretation of MABA endpoints improved with technical experience, The MABA is a simple, rapid, low-cost, appropriate technology which does not require expensive instrumentation and which makes use of a nontoxic, temperature-stable reagent.