A new method based on acoustic impedance measurements for quartz immunosensors
A new method based on acoustic impedance measurements for quartz immunosensors
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DOI：
10.1016/s0925-4005(97)00155-x
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发表时间：
1997-09-01
影响因子：
8.4
通讯作者：
Patat, F
中科院分区：
文献类型：
--
作者：
Schmitt, N;Tessier, L;Patat, F
研制了一种用于特异性检测免疫相互作用的重量石英传感器。抗体通过定向共价偶联固定到功能化石英换能器上。研究免疫传感器响应的实验装置由仅允许提取免疫信号的差分测量系统组成。开发了一个单元和一个合适的计算机程序，用于采集和处理数据，以克服这些声传感器遇到的缺点。两个加载谐振器（免疫传感器和参考）的谐振频率和品质因数（即谐振参数）的变化与加载材料的剪切声阻抗的变化有关。该原始阻抗分析显示了刚性质量存款与界面粘性载荷的变化。(C)1997年Elsevier Science S.A.
A gravimetric quartz sensor has been developed for the specific detection of immunological interactions. Antibodies were immobilized through oriented covalent coupling onto the functionalized quartz transducer. The experimental set-up, to study the immunosensor response, consists of a differential measurement system that allows extraction of only the immunological signal. A cell and a suitable computer program for acquisition and treatment of data were developed to overcome the shortcomings encountered with these acoustic sensors. The variations in resonant frequency and quality factor, i.e. the resonance parameters, of two loaded resonators (immunosensor and reference), are related to the variations in the shear acoustic impedance of the loading materials. This original impedance analysis shows the changes in rigid mass deposit in contrast to interfacial viscous load. (C) 1997 Elsevier Science S.A.