Cell cultures of neuroblasts from rat olfactory epithelium that show odorant responses.
Cell cultures of neuroblasts from rat olfactory epithelium that show odorant responses.
复制标题
来自大鼠嗅上皮的神经母细胞的细胞培养物显示出气味反应。
DOI：
10.1073/pnas.86.5.1703
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发表时间：
1989
影响因子：
11.1
通讯作者：
R. Swerdlow
中科院分区：
文献类型：
--
作者：
H. Coon;F. Curcio;K. Sakaguchi;M. Brandi;R. Swerdlow
我们已经开发了一种程序，允许从5- 7日龄大鼠的嗅上皮中分离和繁殖克隆细胞培养物，这些克隆细胞似乎代表了在整个生命中重新填充嗅上皮中感觉神经元的神经母细胞。我们在这里报道的细胞系合成神经元特异性烯醇化酶，这是一种神经元标志物，43-kDa生长相关蛋白，一种与神经元生长锥相关的蛋白质，以及肌肽，一种可能的嗅觉神经递质。在两个细胞系中，我们发现暴露于亚微摩尔浓度的化学气味剂后，cAMP积累的剂量依赖性。这两种细胞系在与六种通常用作测试气味剂的化学物质进行测试时显示出不同的气味特异性模式。我们预计这些和类似的衍生细胞系将在研究神经发生和嗅觉方面证明是有价值的。
We have developed procedures that permit isolation and propagation of clonal cell cultures from the olfactory epithelium of the 5- to 7-day-old rat that appear to represent the neuroblasts that repopulate the sensory neurons in the olfactory epithelium throughout life. The cell lines we report here synthesize neuron-specific enolase, which is a neuron marker, 43-kDa growth-associated protein, a protein associated with neuronal growth cones, and carnosine, a possible olfactory neurotransmitter. In two of the cell lines we have found dose-dependent cAMP accumulation following exposure to submicromolar concentrations of chemical odorants in the medium. These two cell lines show different patterns of odorant specificity when tested against a panel of six chemicals commonly used as test odorants. We anticipate that these and similarly derived cell lines will prove valuable in studying aspects of neurogenesis and olfaction.