Crystal structure of type VI effector Tse1 from Pseudomonas aeruginosa
Crystal structure of type VI effector Tse1 from Pseudomonas aeruginosa
复制标题
铜绿假单胞菌 VI 型效应子 Tse1 的晶体结构
DOI：
10.1016/j.febslet.2012.06.036
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发表时间：
2012-09-21
期刊：
影响因子：
3.5
通讯作者：
Dong, Yu-Hui
中科院分区：
文献类型：
--
作者：
Zhang, Heng;Gao, Zeng-Qiang;Dong, Yu-Hui
VI型分泌系统（T6SS）在种间竞争中具有新兴的作用。为了在防御其他生物体方面具有优势，铜绿假单胞菌中的该系统将肽聚糖酰胺酶（Tse1）递送到竞争对手的周质空间。细菌还产生一种免疫蛋白（Tsi1），以保护自身免受Tse1造成的损害。Tsi1与Tse1直接相互作用。我们报告说，Tse1的晶体结构显示一个共同的蛋白质折叠。引人注目的是，我们的结构表明，催化三联体中的第三个残基可能是新的，因为这种残基类型以前没有观察到。(C)2012年欧洲生物化学学会联合会。Elsevier B.V.出版，保留所有权利。
The type VI secretion systems (T6SS) have emerging roles in interspecies competition. In order to have an advantage in defense against other organisms, this system in Pseudomonas aeruginosa delivers a peptidoglycan amidase (Tse1) to the periplasmic space of a competitor. An immune protein (Tsi1) is also produced by the bacterium to protect itself from damage caused by Tse1. Tsi1 directly interacts with Tse1. We report that the crystal structure of Tse1 displays a common CHAP protein fold. Strikingly, our structures showed that the third residue in the catalytic triad may be novel as this residue type has not been observed previously. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.