Evidence for a circadian rhythm of insulin secretion
Evidence for a circadian rhythm of insulin secretion
复制标题
DOI：
10.1152/ajpendo.1996.271.2.e246
复制
发表时间：
1996-08-01
影响因子：
5.1
通讯作者：
Chen, XH
中科院分区：
文献类型：
--
作者：
Boden, G;Ruiz, J;Chen, XH
研究了健康志愿者在三种不同水平胰岛素水平下的胰岛素分泌。将血浆葡萄糖钳制在类似于5、类似于8.8和类似于12.6 mM下68 h。测定血清胰岛素浓度和胰岛素分泌率（ISR），后者通过血浆C肽浓度的去卷积。在所有三种葡萄糖浓度下，ISR的节律模式仅被确定（用一阶傅立叶变换），但在12.6 mM时最清楚。ISR和血清胰岛素浓度以昼夜节律（类似于24 h）变化，从午夜至6 A.ryr.之间的最低点开始增加。并在中午至下午6点之间达到峰值。在12.6 mM高血糖时，由于胰岛素清除率下降（从1.55降至0.5 l/min，P < 0.01），胰岛素浓度周期的振幅大于ISR周期的振幅（+/-13.0 vs. +/-8.7%）。血浆褪黑激素水平（亮-暗节律性的标志物）以相反的方向变化，即，当ISR触底时，它们达到峰值，当ISR达到峰值时，它们又触底。我们的结论是，正常人受试者有一个昼夜节律的胰岛素分泌，这变得更加明显，与ISR上升，昼夜变化ISR，在白天上升，在夜间下降：可能是一个解释的良好建立的观察，葡萄糖耐量和胰岛素对葡萄糖和膳食的反应是在早上比晚上更高。
Insulin secretion was studied in healthy volunteers at three different levels of glycemia. Plasma glucose was clamped at similar to 5, similar to 8.8 and similar to 12.6 mM for 68 h. Measured were serum insulin concentration and insulin secretion rates (ISR), the latter by deconvolution of plasma C-peptide concentration. Rhythmic patterns of ISR mere identified (with a refined first-order Fourier transform) at all three glucose concentrations tested but were most clearly seen at 12.6 mM. ISR and serum insulin concentration changed in a circadian (similar to 24 h) rhythm, increasing from a nadir between midnight and 6 A.ryr. and reaching a peak between noon and 6 P.M. At 12.6 mM hyperglycemia, the amplitude of the insulin concentration cycles was greater than that of the ISR cycles (+/-13.0 vs. +/-8.7%) due to a decrease in insulin clearance (from 1.55 to 0.5 l/min, P < 0.01). Plasma melatonin levels (a marker of light-dark rhythmicity) changed in the opposite direction, i.e., they peaked when ISR bottomed and bottomed when ISR peaked. We concluded that normal human subjects have a circadian rhythm of insulin secretion, which becomes more apparent with rising ISR, and that circadian changes in ISR, rising during the day and falling during the night: may be one explanation for the well-established observation that glucose tolerance and insulin responses to glucose and meals are higher in the morning than at night.