Effects of water stress on major photosystem II gene expression and protein metabolism in barley leaves
Effects of water stress on major photosystem II gene expression and protein metabolism in barley leaves
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DOI：
10.1111/j.1399-3054.2005.00577.x
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发表时间：
2005-12-01
影响因子：
6.4
通讯作者：
Lin, HH
中科院分区：
文献类型：
--
作者：
Yuan, S;Liu, WJ;Lin, HH
虽然人们很早就认识到植物的水分亏缺会降低光系统(PS)II的mRNAs和蛋白质，但其背后的详细机制还没有被彻底阐明。本研究以大麦叶片为材料，研究了水分胁迫对大麦主要PSII基因的降解及PSII蛋白的解离和迁移的影响。结果表明：(1)随着水分胁迫的加剧，主要PSII的mRNAs和蛋白质的稳态水平下降，降解加剧；在重度水分胁迫下，D1和D2蛋白的半衰期从12-14h缩短到7-8h，PSBA和psbD的半衰期从16-10h减少到6-10h；(2)水分胁迫下PSII的单体增加。严重的水分胁迫加速了PSII的周转，抑制了PSII的活性。
Although it has long been recognized that water deficit in plants reduces photosystem (PS) II mRNAs and proteins, the detailed mechanisms behind this have not been thoroughly elucidated. In the present study, effects of water stress in barley leaves on degradation of major PSII mRNA and dissociation and migration of PSII proteins were investigated. The results indicated that (1) the steady-state levels of major PSII mRNAs and proteins declined with increasing water stress, as a consequence of increased degradation; under severe water stress, the half-lives of D1 and D2 proteins decreased from 12-14 h to 7-8 h and the half-lives of psbA and psbD mRNA decreased from above 16 to 6-10 h; (2) monomerization of PSII were increased during water stress. Severe water stress accelerated turnover of PSII and inhibited PSII activities.