Quantitative Analysis of Photobodies.
Quantitative Analysis of Photobodies.
复制标题
DOI：
10.1007/978-1-4939-9612-4_10
复制
发表时间：
2019
影响因子：
--
通讯作者：
Chan Yul Yoo;Desiree Williams;Meng Chen
中科院分区：
文献类型：
--
作者：
Chan Yul Yoo;Desiree Williams;Meng Chen
光体是无膜的亚核细胞器，含有红色和远红色光感受器，光敏色素。光体生物合成被认为在早期光信号事件中起重要作用。光体的大小和数量是高度动态的，响应于光的质量和数量，并与光敏色素介导的幼苗形态建成密切相关。在这里，我们提供了一个详细的协议，用于表征的三维形态的光体，包括样品制备，荧光显微镜，和图像分析。虽然这种方法最初是为了表征光体而开发的，但它可以用于分析其他无膜或膜结合的亚细胞器。
Photobodies are membraneless subnuclear organelles that contain the red and far-red photoreceptors, phytochromes. Photobody biogenesis has been postulated to play important roles in early light signaling events. The size and number of photobodies are highly dynamic in response to the quality and quantity of light and correlated tightly with phytochrome-mediated seedling morphogenesis. Here, we provide a detailed protocol for characterization of the three-dimensional morphology of photobodies, including sample preparation, fluorescence microscopy, and image analysis. Although this method was developed initially for characterizing photobodies, it can be adopted to analyze other membraneless or membrane-bound subcellular organelles.