Resveratrol and curcumin ameliorate di-(2-ethylhexyl) phthalate induced testicular injury in rats
Resveratrol and curcumin ameliorate di-(2-ethylhexyl) phthalate induced testicular injury in rats
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DOI：
10.1016/j.ygcen.2015.09.006
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发表时间：
2016-01-01
影响因子：
2.7
通讯作者：
Ali, Bassam Mohamed
中科院分区：
文献类型：
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作者：
Abd El-Fattah, Amal Ahmed;Fahim, Atef Tadros;Ali, Bassam Mohamed
本研究旨在评价白藜芦醇和姜黄素对邻苯二甲酸二（2-乙基己基）酯（DEHP）诱导的睾丸氧化损伤的保护作用。将雄性Wistar大鼠分为6组; 3组每日经口给予DEHP（2 g/kg BW）45天，以诱导睾丸损伤。其中两组在给予DEHP之前30天和之后45天口服白藜芦醇（80 mg/kg BW）或姜黄素（200 mg/kg BW）。还包括溶剂处理的对照组。另外两组大鼠单独接受白藜芦醇或姜黄素。氧化损伤的总抗氧化能力（TAC）和谷胱甘肽（GSH）的水平下降，并增加丙二醛（MDA）的水平在DEHP给药大鼠的睾丸中观察。血清睾酮水平以及睾丸标志酶活性;酸性和碱性磷酸酶（ACP和ALP）和乳酸脱氢酶（LDH）显示严重下降。与对照组相比，DEHP给药导致Nrf 2、HO-1、HSP 60、HSP 70和HSP 90的睾丸基因表达水平显著增加，c-Kit蛋白显著降低。组织病理学观察为生化和分子分析提供了证据。这些DEHP诱导的病理变化减弱预处理与白藜芦醇和姜黄素。结论：白藜芦醇和姜黄素预处理可使DEHP所致的睾丸生化、分子和组织结构损伤得到恢复。这些化合物的化学保护作用可能是由于其固有的抗氧化特性沿着增加Nrf 2、HSP 60、HSP 70和HSP 90基因表达水平，因此可能是对抗DEHP诱导的睾丸功能障碍的有用的潜在工具。(C)2015 Elsevier Inc. All rights reserved.
The present study aimed to evaluate the protective role of resveratrol and curcumin on oxidative testicular damage induced by di-(2-ethylhexyl) phthalate (DEHP). Male Wistar rats were divided into six groups; three groups received oral daily doses of DEHP (2 g/kg BW) for 45 days to induce testicular injury. Two of these groups received either resveratrol (80 mg/kg BW) or curcumin (200 mg/kg BW) orally for 30 days before and 45 days after DEHP administration. A vehicle-treated control group was also included. Another two groups of rats received either resveratrol or curcumin alone. Oxidative damage was observed by decreased levels of total antioxidant capacity (TAC) and glutathione (GSH) and increased malondialdehyde (MDA) level in the testes of DEHP-administered rats. Serum testosterone level as well as testicular marker enzymes activities; acid and alkaline phosphatases (ACP and ALP) and lactate dehydrogenase (LDH) showed severe declines. DEHP administration caused significant increases in the testicular gene expression levels of Nrf2, HO-1, HSP60, HSP70 and HSP90 as well as a significant decrease in c-Kit protein when compared with the control group. Histopathological observations provided evidence for the biochemical and molecular analysis. These DEHP-induced pathological alterations were attenuated by pretreatment with resveratrol and curcumin. We conclude that DEHP-induced injuries in biochemical, molecular and histological structure of testis were recovered by pretreatment with resveratrol and curcumin. The chemoprotective effects of these compounds may be due to their intrinsic antioxidant properties along with boosting Nrf2, HSP 60, HSP 70 and HSP 90 gene expression levels and as such may be useful potential tools in combating DEHP-induced testicular dysfunction. (C) 2015 Elsevier Inc. All rights reserved.