Gelatin Nanoparticle-Injectable Platelet-Rich Fibrin Double Network Hydrogels with Local Adaptability and Bioactivity for Enhanced Osteogenesis
Gelatin Nanoparticle-Injectable Platelet-Rich Fibrin Double Network Hydrogels with Local Adaptability and Bioactivity for Enhanced Osteogenesis
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具有局部适应性和增强成骨作用的生物活性的明胶纳米颗粒注射富含血小板纤维蛋白双网络水凝胶
DOI：
10.1002/adhm.201901469
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发表时间：
2020-01-29
影响因子：
10
通讯作者：
Chen, Tao
中科院分区：
文献类型：
--
作者：
Mu, Zhixiang;Chen, Kaiwen;Chen, Tao
骨愈合是一个受趋化因子、生长因子等生化信号和细胞外基质的地形、力学特征或机械刺激等生物物理信号调控的动态过程。因此，一种机械韧性和生物活性的水凝胶是基于自体注射富血小板纤维蛋白（iPRF）修饰的明胶纳米颗粒（GNPs）。该复合水凝胶表现出双网络（DN）机制，其中纤维蛋白的共价网络维持材料的完整性，而GNPs的自组装胶体网络在加载时消散力。采用兔窦增强模型研究DN水凝胶的生物活性和成骨能力。DN水凝胶适应骨缺损局部环境的复杂性，即适应骨缺损的不规则形状，承受动物呼吸过程中上颌窦内形成的压力。DN水凝胶也被证明可以吸收和延长iPRF产生的生物活性生长因子的释放，这可能有助于在窦内观察到的早期血管生成和成骨。这种适应性强且具有生物活性的DN水凝胶可以通过长期维持骨量和承受功能性机械刺激来增强骨再生，从而治疗复杂骨缺损。
Bone healing is a dynamic process regulated by biochemical signals such as chemokines and growth factors, and biophysical signals such as topographical and mechanical features of extracellular matrix or mechanical stimuli. Hereby, a mechanically tough and bioactive hydrogel based on autologous injectable platelet-rich fibrin (iPRF) modified with gelatin nanoparticles (GNPs) is developed. This composite hydrogel demonstrates a double network (DN) mechanism, wherein covalent network of fibrin serves to maintain material integrity, and self-assembled colloidal network of GNPs dissipates force upon loading. A rabbit sinus augmentation model is used to investigate the bioactivity and osteogenesis capacity of the DN hydrogels. The DN hydrogels adapt to the local environmental complexity of bone defects, i.e., accommodate the irregular shape of the defects and withstand the pressure formed in the maxillary sinus during animal's respiration process. The DN hydrogel is also demonstrated to absorb and prolong the release of the bioactive growth factors stemming from iPRF, which could have contributed to the early angiogenesis and osteogenesis observed inside the sinus. This adaptable and bioactive DN hydrogel can achieve enhanced bone regeneration in treating complex bone defects by maintaining long-term bone mass and withstanding the functional mechanical stimuli.