Mitogen-activated protein kinase cascades in Vitis vinifera.
Mitogen-activated protein kinase cascades in Vitis vinifera.
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DOI：
10.3389/fpls.2015.00556
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发表时间：
2015
影响因子：
5.6
通讯作者：
Kılıçkaya O
中科院分区：
文献类型：
--
作者：
Çakır B;Kılıçkaya O
蛋白质磷酸化是调控细胞功能的重要机制之一。丝裂原活化蛋白激酶（MAPK）是真核生物中的通用信号分子，其介导细胞外信号的细胞内传递，导致诱导适当的细胞应答。MAPK级联由四个蛋白激酶模块组成：MAPKKK激酶（MAPKKKKs）、MAPKK激酶（MAPKKKs）、MAPK激酶（MAPKKs）和MAPK。在植物中，MAPKs在响应非生物胁迫、创伤和激素时以及在植物病原体相互作用和细胞分裂期间被激活。在这份报告中，我们进行了一个完整的清单的MAPK级联基因在葡萄属葡萄，全基因组测序。通过与MAPK、MAPK激酶、MAPK激酶和MAPK激酶成员的比较，我们发现葡萄属葡萄中存在14个MAPK、5个MAPK、62个MAPK和7个MAPK激酶激酶。我们确定了葡萄在不同物种中推定的MAPK的直系同源物，以及在各种组织中对应于MAPK级联成员的EST。这项工作代表了葡萄中MAPK级联的第一个完整清单，并有助于阐明葡萄中这些蛋白质的生物学和生理功能。
Protein phosphorylation is one of the most important mechanisms to control cellular functions in response to external and endogenous signals. Mitogen-activated protein kinases (MAPK) are universal signaling molecules in eukaryotes that mediate the intracellular transmission of extracellular signals resulting in the induction of appropriate cellular responses. MAPK cascades are composed of four protein kinase modules: MAPKKK kinases (MAPKKKKs), MAPKK kinases (MAPKKKs), MAPK kinases (MAPKKs), and MAPKs. In plants, MAPKs are activated in response to abiotic stresses, wounding, and hormones, and during plant pathogen interactions and cell division. In this report, we performed a complete inventory of MAPK cascades genes in Vitis vinifera, the whole genome of which has been sequenced. By comparison with MAPK, MAPK kinases, MAPK kinase kinases and MAPK kinase kinase kinase kinase members of Arabidopsis thaliana, we revealed the existence of 14 MAPKs, 5 MAPKKs, 62 MAPKKKs, and 7 MAPKKKKs in Vitis vinifera. We identified orthologs of V. vinifera putative MAPKs in different species, and ESTs corresponding to members of MAPK cascades in various tissues. This work represents the first complete inventory of MAPK cascades in V. vinifera and could help elucidate the biological and physiological functions of these proteins in V. vinifera.