Development and use of an animal model to study post-traumatic stiffness and contracture of the elbow
Development and use of an animal model to study post-traumatic stiffness and contracture of the elbow
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DOI：
10.1002/jor.22981
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发表时间：
2016-02-01
影响因子：
2.8
通讯作者：
Galatz, Leesa M.
中科院分区：
文献类型：
--
作者：
Lake, Spencer P.;Castile, Ryan M.;Galatz, Leesa M.
手肘创伤后关节僵硬（PTJS）是一种使人衰弱的疾病，带来了独特的治疗挑战。虽然以前的研究涉及PTJS中的包膜组织，但关于这种情况的发展和进展仍然未知。本研究的目的是建立一种创伤后肘关节挛缩的动物模型，并评估其在PTJS病因学研究中的潜力。由于与人类肘关节的解剖学和功能相似性，Long-Evans大鼠被确定为最适合开发的种属/品种。采用两种不同严重程度的手术方案来复制肘关节半脱位/脱位损伤中观察到的软组织损伤，包括前囊切开术和外侧副韧带切断术，随后进行6周的单侧关节固定。处死后，屈伸机械关节测试表明，与对照组和假手术组动物相比，受伤固定肢体的活动范围减少，僵硬度增加，其中功能影响与损伤严重程度相关。组织学评价显示，受伤肢体的细胞结构、粘附和包膜组织厚度增加，与临床证据一致。据我们所知，这是第一个能够研究解剖学和生物力学复杂肘关节独特挑战的动物模型。未来的研究将使用这种动物模型来研究PTJS的机制。(c)2015骨科研究学会。出版社：Wiley Periodicals，Inc. J Orthop Res 34：354-364，2016.
Post-traumatic joint stiffness (PTJS) of the elbow is a debilitating condition that poses unique treatment challenges. While previous research has implicated capsular tissue in PTJS, much regarding the development and progression of this condition remains unknown. The objective of this study was to develop an animal model of post-traumatic elbow contracture and evaluate its potential for studying the etiology of PTJS. The Long-Evans rat was identified as the most appropriate species/breed for development due to anatomical and functional similarities to the human elbow joint. Two surgical protocols of varying severity were utilized to replicate soft tissue damage seen in elbow subluxation/dislocation injuries, including anterior capsulotomy and lateral collateral ligament transection, followed by 6 weeks of unilateral joint immobilization. Following sacrifice, flexion-extension mechanical joint testing demonstrated decreased range-of-motion and increased stiffness for injured-immobilized limbs compared to control and sham animals, where functional impact correlated with severity of injury. Histological evaluation showed increased cellularity, adhesion, and thickness of capsule tissue in injured limbs, consistent with clinical evidence. To our knowledge, this is the first animal model capable of examining challenges unique to the anatomically and biomechanically complex elbow joint. Future studies will use this animal model to investigate mechanisms responsible for PTJS. (c) 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 34:354-364, 2016.