BIOSYNTHESIS OF SEX-PHEROMONE COMPONENTS AND GLYCEROLIPID PRECURSORS FROM SODIUM [1-C-14]LABELLED ACETATE IN REDBANDED LEAFROLLER MOTH
BIOSYNTHESIS OF SEX-PHEROMONE COMPONENTS AND GLYCEROLIPID PRECURSORS FROM SODIUM [1-C-14]LABELLED ACETATE IN REDBANDED LEAFROLLER MOTH
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DOI：
10.1007/bf00994228
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发表时间：
1984-01-01
影响因子：
2.3
通讯作者：
ROELOFS, WL
中科院分区：
文献类型：
--
作者：
BJOSTAD, LB;ROELOFS, WL
将[1- 14 C]乙酸钠在水-二甲基亚砜（1：1）中局部应用于A. velutiana。将放射性标记掺入信息素组分（Z）-11-十四烯乙酸酯和（E）-11-十四烯乙酸酯中，并且还掺入三酰基甘油、二酰基甘油、乙醇胺磷脂和胆碱磷脂中。在三酰甘油中，放射性标记出现在（Z）-11-十四碳烯酸、（E）-11-十四碳烯酸、十四酸、十六酸和十八酸中。在胆碱磷脂中，相同的酰基部分掺入放射性标记，但水平较低。在二酰基甘油和乙醇胺磷脂中，仅十六烷酸酯和十八烷酸酯中的放射性标记高于检测限。在施用[1- 14 C]乙酸钠后的不同时间，标记的（Z）-11-十四烯乙酸酯和（E）-11-十四烯乙酸酯的相对比例变化很小，但标记的脂肪酰基部分在三酰甘油和胆碱磷脂中的相对比例变化显著。8分钟后，三酰基甘油将约等量的放射性标记掺入（Z）-11-十四烷酸酯、（E）-11-十四烯酸酯和十四烷酸酯中。随着孵育时间的增加，三酰基甘油中放射性标记的（E）-11-十四烯酸酯的积累比例高于（Z）-11-十四烯酸酯，放射性标记的十四酸酯的积累比例低于（Z）-11-十四烯酸酯。在胆碱磷脂中，在孵育的所有时间点，掺入（Z）-11-十四烯酸酯的放射性标记量较小，但高于检测限，（E）-11-十四烯酸酯和十四酸酯中的放射性标记量较小，通常低于检测限。在三酰甘油和胆碱磷脂中，放射性标记的十六烷酸的相对比例随时间的推移而下降，十八烷酸的相对比例增加。
Sodium [1-14C]acetate in water-dimethyl sulfoxide (1:1) was applied topically to sex pheromone glands of A. velutiana. Radiolabel was incorporated into the pheromone components (Z)-11-tetradecenyl acetate and (E)-11-tetradecenyl acetate, and also into triacylglycerols, diacylglycerols, ethanolamine phosphatides, and choline phosphatides. In the triacylglycerols, radiolabel appeared in (Z)-11-tetradecenoate, (E)-11-tetradecenoate, tetradecanoate, hexadecanoate and octadecanoate. In the choline phosphatides, the same acyl moieties incorporated radiolabel but at lower levels. In the diacylglycerols and ethanolamine phosphatides, only the radiolabel in hexadecanoate and octadecanoate was above the limit of detection. At different times following application of sodium [1-14C]acetate, the relative proportions of labeled (Z)-11-tetradecenyl acetate and (E)-11-tetradecenyl acetate changed very little, but the relative proportions of labeled fatty acyl moieties in the triacylglycerols and choline phosphatides changed markedly. After 8 min, triacylglycerols had incorporated about equal amounts of radiolabel into (Z)-11-tetradecanoate, (E)-11-tetradecenoate and tetradecanoate. As the incubation time was increased, triacylglycerols accumulated proportionately more radiolabeled (E)-11-tetradecenoate than (Z)-11-tetradecenoate, and accumulated proportionately less radiolabeled tetradecanoate. In the choline phosphatides, at all times of incubation the amount of radiolabel incorporated into (Z)-11-tetradecenoate was small but above the limit of detection, and the amounts of radiolabel in (E)-11-tetradecenoate and tetradecanoate were smaller and often below the limit of detection. In both the triacylglycerols and the choline phosphatides, the relative proportion of radiolabeled hexadecanoate decreased with time, and that of octadecanoate increased.