Protochlorophyllide a: A Comprehensive Photophysical Picture
Protochlorophyllide a: A Comprehensive Photophysical Picture
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DOI：
10.1002/cphc.200800536
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发表时间：
2009-01-12
期刊：
影响因子：
2.9
通讯作者：
Popp, Juergen
中科院分区：
文献类型：
--
作者：
Dietzek, Benjamin;Tschierlei, Stefanie;Popp, Juergen
利用泵浦-探测光谱法研究了叶绿素生物合成的前体物质原叶绿素a的光化学性质。在激发到最低的躺Q-带的光诱导的变化矿石记录在可见光和近红外区域之间的500和1000 nm之间的宽范围的探测波长。在激发之后，观察到与激发态表面上的Franck-Condon区域外的运动相关的初始超快450 Is过程;因此直接解开先前基于时间分辨荧光测量的建议（ChemPhysChem 2006，7，1727-1733）。此外，数据揭示了一个以前隐藏的光中间体，其形成纳秒时间尺度匹配的整体荧光衰减，并被分配到一个三重态。这一发现对NADPH光化学的影响。原叶绿素氧化还原酶（POR）矿石讨论。
The photochemistry of protochlorophyllide a, a precursor in the biosynthesis of chlorophyll and substrate of the light regulated enzyme protochlorophyllide oxidoreductase, is investigated by pump-probe spectroscopy. Upon excitation into the lowest lying Q-band the light induced changes ore recorded over a wide range of probe wavelengths in the visible and near-IR region between 500 and 1000 nm. Following excitation, an initial ultrafast 450 Is process is observed related to the motion out of the Franck-Condon region on the excited state surface; thus directly unraveling previous suggestions based on time-resolved fluorescence measurements (ChemPhysChem 2006, 7, 1727-1733). Furthermore, the data reveals a previously concealed photointermediate, whose formation on a nanosecond timescale matches the overall fluorescence decay and is assigned to a triplet state. The implications of this finding with respect to the photochemistry of NADPH.-protochlorophyllide oxidoreductase (POR) ore discussed.