APPARENT VISCOSITY AND CORTICAL TENSION OF BLOOD GRANULOCYTES DETERMINED BY MICROPIPET ASPIRATION
APPARENT VISCOSITY AND CORTICAL TENSION OF BLOOD GRANULOCYTES DETERMINED BY MICROPIPET ASPIRATION
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DOI：
10.1016/s0006-3495(89)82660-8
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发表时间：
1989-07-01
影响因子：
3.4
通讯作者：
YEUNG, A
中科院分区：
文献类型：
--
作者：
EVANS, E;YEUNG, A
已经研究了在各种抽吸压力下单个血液粒细胞进入小口径微量移液管的连续变形和入口流动，以确定细胞内容物的表观粘度，并估计在细胞表面附近的皮质层中的耗散对总粘性流动阻力的贡献程度。用宽范围的吸量管尺寸（2.0-7.5 μ m）和抽吸压力（102-104达因/厘米2）进行实验以检查入口流的细节。结果表明，细胞的外皮层保持约0.035dyn/cm的小的持续张力。张力产生阈值压力，低于该阈值压力，细胞将不会进入移液管。这些细胞的表面质膜似乎建立了表面扩张的上限，在显微镜下的“褶边”和“褶皱”被拉直后达到该上限。用小移液管（2.7 μ m）抽吸细胞。在一篇配套论文中介绍了一个理论模型，（Yeung，A.，和E.埃文斯. 1989. Biophys. J. 56：139-149）分析入口流动响应对移液管尺寸和抽吸压力的关系，以估计细胞内部的表观粘度和皮质流动阻力与来自细胞内部的流动阻力的比率。发现表观粘度强烈地依赖于温度，其值约为2 × 10 - 4。23 ℃时为103泊，较低值为1 × 103泊。在37 ℃时为103泊，但在10 ℃以下超过104泊的极大值。由于细胞反应的分散性，不可能准确地建立皮质中流动阻力与细胞内流动阻力的特征比率;然而，数据显示皮质对总流动阻力没有显著贡献。
Continous deformation and entry flow of single blood granulocytes into small caliber micropipets at various suction pressures have been studied to determine an apparent viscosity for the cell contents and to estimate the extent that dissipation in a cortical layer adjacent to the cell surface contributes to the total viscous flow resistance. Experiments were carried out with a wide range of pipet sizes (2.0-7.5 .mu.m) and suction pressures (102-104 dyn/cm2) to examine the details of the entry flow. The results show that the outer cortex of the cell maintains a small persistent tension of .apprx.0.035 dyn/cm. The tension creates a threshold pressure below which the cell will not enter the pipet. The superficial plasma membrane of these cells appears to establish an upper limit to surface dilation which is reached after microscopic "ruffles" and "folds" have been pulled smooth. With aspiration of cells by small pipets (2.7 .mu.m). With a theoretical model introduced in a companion paper, (Yeung, A., and E. Evans. 1989. Biophys. J. 56:139-149) the entry flow response versus pipet size and suction pressure was analyzed to estimate the apparent viscosity of the cell interior and the ratio of cortical flow resistance to flow resistance from the cell interior. The apparent viscosity was found to depend strongly on temperature with values on the order of 2 .times. 103 poise at 23.degree.C, lower values of 1 .times. 103 poise at 37.degree.C, but extremely large values in excess of 104 poise below 10.degree.C. Because of scatter in cell response, it was not possible to accurately establish the characteristic ratio for flow resistance in the cortex to that inside the cell; however, the data showed that the cortex does not contribute significantly to the total flow resistance.