Cloning and tissue distribution of two Na+/H+ exchangers from the Malpighian tubules of Aedes aegypti.
Cloning and tissue distribution of two Na+/H+ exchangers from the Malpighian tubules of Aedes aegypti.
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埃及伊蚊马氏管中两种 Na /H 交换体的克隆和组织分布。
DOI：
10.1002/arch.10057
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发表时间：
2002
影响因子：
2.2
通讯作者：
Petzel,DH
中科院分区：
文献类型：
--
作者：
Hart,SJ;Knezetic,JA;Petzel,DH
据信，蚊子马尔皮基管 (MT) 细胞顶膜上的 Na+/H+ 交换器 (NHE) 参与了吸血蚊子重要的 Na+ 和液体分泌。本研究介绍了编码埃及伊蚊 MT NHE 的两种 cDNA 的分子克隆、一级结构和组织分布。使用逆转录聚合酶链式反应 (RT-PCR) 和 5' 和 3' cDNA 末端快速扩增 (RACE) 从蚊子 MT 总 RNA 中获得 cDNA 序列。这两个序列编码 678 和 1,179 个氨基酸的蛋白质，计算分子量分别为 74,473 和 130,276。当将 678 个氨基酸的蛋白质与另一个蛋白质的前 678 个氨基酸进行比较时，两个克隆彼此显示出 98% 的同一性。它们还表现出与黑腹果蝇 NHE 的高度同一性。水疗分析显示，虽然两个克隆都有 10-13 个跨膜片段，但 1,179 个氨基酸的蛋白质具有广泛的羧基末端，而 678 个氨基酸的蛋白质具有极短的羧基末端。 RT-PCR 分析表明，除了吸血前后的成虫阶段外，这两个克隆在幼虫和蛹阶段的蚊子马氏小管中都有表达。在成年蚊子卵巢、中肠和后肠中也检测到了这两个克隆的表达。拱。昆虫生物化学。生理学。 51:121–135, 2002。© 2002 Wiley‐Liss, Inc.
A Na+/H+exchanger (NHE) on the apical membrane of mosquito Malpighian tubule (MT) cells is believed to participate in the blood‐feeding mosquito’s vital secretion of Na+and fluid. This study presents the molecular cloning, primary structure, and tissue distribution of two cDNAs encodingAedes aegyptimosquito MT NHEs. The cDNA sequences were obtained from mosquito MT total RNA using reverse transcription‐polymerase chain reaction (RT‐PCR) and 5′ and 3′ rapid amplification of cDNA ends (RACE). The two sequences encode proteins of 678 and 1,179 amino acids with calculated molecular weights of 74,473 and 130,276, respectively. When comparing the 678 amino acid protein to the first 678 amino acids of the other protein, the two clones show 98% identity to each other. They also exhibit high identity toDrosophila melanogasterNHEs. Hydropathy analysis reveals that while both clones have 10–13 transmembrane segments, the 1,179 amino acid protein has an extensive carboxy terminus while the 678 amino acid protein has an extremely short carboxy terminus. RT‐PCR analysis shows that both clones are expressed in the mosquito Malpighian tubules at the larval and pupal stages, in addition to the adult stage before and after blood‐feeding. Expression of both clones was also detected in adult mosquito ovaries, midguts, and hindguts. Arch. Insect Biochem. Physiol. 51:121–135, 2002. © 2002 Wiley‐Liss, Inc.