Rev regulates translation of human immunodeficiency virus type 1 RNAs
Rev regulates translation of human immunodeficiency virus type 1 RNAs
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DOI：
10.1099/vir.0.007963-0
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发表时间：
2009-05-01
影响因子：
3.8
通讯作者：
Lever, Andrew M. L.
中科院分区：
文献类型：
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作者：
Groom, Harriet C. T.;Anderson, Emma C.;Lever, Andrew M. L.
全长人类免疫缺陷病毒1型（HIV-1）RNA作为mRNA（编码Gag和Gag-Pol多聚蛋白）和基因组RNA发挥作用。这种RNA的翻译必须严格控制，以允许在转换为颗粒生产之前进行足够的蛋白质合成。病毒蛋白Rev刺激含有Rev反应元件的未剪接的HIV-1 RNA的核输出，但也可能刺激这些RNA的翻译。我们先前在HIV-1 RNA的5'非翻译区鉴定了一个额外的Rev结合位点。我们发现，Rev在高浓度下非特异性地抑制翻译，并在体外中等浓度下刺激HIV-1 RNA的翻译。刺激依赖于5'非翻译区内Rev结合位点的存在，而不是Rev反应元件。在COS-1细胞中，来自HIV-1报告基因的翻译通过Rev的共表达而特异性增加。
Full-length human immunodeficiency virus type 1 (HIV-1) RNA acts as both mRNA, encoding Gag and Gag-Pol polyproteins, and genomic RNA. Translation of this RNA must be tightly controlled to allow sufficient protein synthesis prior to a switch to particle production. The viral protein Rev stimulates nuclear export of unspliced HIV-1 RNAs containing the Rev response element, but may also stimulate translation of these RNAs. We previously identified an additional Rev binding site in the 5' untranslated region of the HIV-1 RNA. We show that Rev inhibits translation non-specifically at high concentrations and stimulates translation of HIV-1 RNAs at intermediate concentrations in vitro. Stimulation is dependent on the presence of the Rev binding site within the 5' untranslated region and not on the Rev response element. In COS-1 cells, translation from an HIV-1 reporter is specifically increased by coexpression of Rev.