Transcriptomic Analysis Reveals the Effects of Microgravity on Rice Calli on Board the Chinese Spaceship Shenzhou 8
Transcriptomic Analysis Reveals the Effects of Microgravity on Rice Calli on Board the Chinese Spaceship Shenzhou 8
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转录组分析揭示微重力对中国神舟八号飞船上水稻愈伤组织的影响
DOI：
10.1007/s12217-018-9633-6
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发表时间：
2018-12-01
影响因子：
1.8
通讯作者：
Cai, Weiming
中科院分区：
文献类型：
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作者：
Jin, Jing;Chen, Haiying;Cai, Weiming
在航天和地面控制实验中，水稻愈伤组织被装载到生物培养箱（SIMBOX）的固定和旋转平台上。在航天器飞行324小时后，SIMBOX中的愈伤组织在空间中被RNAlater固定，并在地面控制实验的同一时间点被固定在地面上。通过将航天控制（F-μg）与1地控制（G-1g）和1地飞行控制（F-1g）进行比较，确定了微重力响应（MR）转录本。使用MapMan分析对955份MR转录本进行分类。这些转录本主要属于以下几类：细胞壁修饰和代谢、糖酵解和三羧酸（TCA）循环、转录因子、蛋白质修饰和降解、激素代谢和信号传导、钙调节、受体样激酶活性和转运蛋白。本文主要研究了微重力对植物细胞壁的影响，探讨了微重力条件下植物细胞壁的变化与细胞生长的关系。
Rice calli were loaded onto the stationary and rotating platforms of a biological incubator (SIMBOX) during spaceflight and ground control experiments. The calli in the SIMBOX were fixed with RNAlater in space after a 324-h spacecraft flight, as well as on the ground at the same time point in a ground control experiment. Microgravity-responsive (MR) transcripts were identified by a comparison of the spaceflight controls (F-μg) with the 1-gground controls (G-1g) and 1-ginflight controls (F-1g). MapMan analysis was used to classify 955 MR transcripts. These transcripts mainly belonged to the following categories: cell wall modification and metabolism, glycolysis and the tricarboxylic acid (TCA) cycle, transcription factors, protein modification and degradation, hormone metabolism and signalling, calcium regulation, receptor-like kinase activity, and transporters. Here, we focused on the effects of microgravity on the plant cell wall and discussed the relationship between the variation in the cell wall and plant cell growth under microgravity.