Broadband phase correction of FT-ICR mass spectra via simultaneous excitation and detection
Broadband phase correction of FT-ICR mass spectra via simultaneous excitation and detection
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DOI：
10.1021/ac049733i
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发表时间：
2004-10-01
影响因子：
7.4
通讯作者：
Marshall, AG
中科院分区：
文献类型：
--
作者：
Beu, SC;Blakney, GT;Marshall, AG
在典型的傅里叶变换离子回旋共振（FT-ICR）质谱中，时间分散的激发和激发与检测之间的延迟导致被检测的时域离子信号中信号相位随频率连续变化。这种信号的复频域频谱是具有相应的不对称峰的吸收模式和色散模式频谱分量的线性组合。因此，通常采用幅度模式光谱显示，以牺牲光谱分辨率为代价获得与相位无关的均匀对称的峰形。在这项工作中，我们实现了同时激发和检测，使傅里叶反卷积能够恢复低场和高场FT-ICR仪器的吸收模式光谱。这些光谱产生的分辨率提高因子接近2.0的最大理论极限，并且相对于传统的等模光谱减少了频率分配误差。傅里叶反褶积过程具有校正由激励带宽上的非均匀功率分布引起的光谱变化的额外好处，以及为解释实验性能提供有用的诊断信息的潜在好处。
In typical Fourier transform ion cyclotron resonance (FT-ICR) mass spectra, temporally dispersed excitation and the delay between excitation and detection result in continuous variation of signal phase with frequency in the detected time-domain ion signal. The complex frequency-domain spectrum of such a signal is a linear combination of absorption- and dispersion-mode spectral components with corresponding asymmetric peaks. For this reason, magnitude-mode spectral display is usually employed to yield a phase-independent uniform and symmetrical peak shape at the expense of spectral resolution. In this work, we implement simultaneous excitation and detection to enable Fourier deconvolution to recover absorption-mode spectra for both low- and high-field FT-ICR instruments. These spectra yield resolving power improvement factors approaching the maximum theoretical limit of 2.0, as well as reduction in frequency assignment errors relative to conventional magnitude-mode spectra. The Fourier deconvolution procedure has the additional benefit of correcting for spectral variation resulting from nonuniform power distribution over the excitation bandwidth and the potential benefit of providing useful diagnostic information for interpretation of experimental performance.